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IHCPlus™ FBXW7 / FBW7 Antibody aa251‑300 Polyclonal IHC,WB LS‑B5634

Catalog Number Size Price
LS-B5634-100 100 µl $485 
Anti-FBXW7 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FBXW7 antibody Western blot of Mouse Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FBXW7 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FBXW7 antibody Western blot of Mouse Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ FBXW7 / FBW7 Antibody aa251‑300 Polyclonal IHC,WB LS‑B5634

Note: This antibody replaces LS-C116100
Antibody:
Rabbit Polyclonal to Mouse FBXW7 / FBW7
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5634-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse FBXW7 / FBW7
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FBW7 antibody LS-B5634 is an unconjugated rabbit polyclonal antibody to FBW7 (FBXW7 ) from mouse, rat and hamster. Validated for IHC and WB.
Target
Mouse FBXW7 / FBW7
Host
Rabbit
Reactivity
Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa251-300 of mouse Fbxw7 (Q8VBV4, NP_536353). Percent identity by BLAST analysis: Mouse, Rat, Hamster, Opossum (100%); Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Zebrafish (92%); Xenopus (85%).
Blocking Peptide
LS-E13424 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5634. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa251-300
Specificity
Mouse FBXW7
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FBXW7 / FBW7
Q969H0 NM_018315 NP_060785.2

Validation

Anti-FBXW7 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-FBXW7 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FBXW7 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXW7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXW7.
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Requested From: United States
Date Requested: 9/23/2019