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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B2909-50 50 µg (0.25 mg/ml) $485 
FBXW7 / FBW7 Antibody - Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ Polyclonal Rabbit anti‑Human FBXW7 / FBW7 Antibody (Internal, IHC, WB) LS‑B2909

IHCPlus™ Polyclonal Rabbit anti‑Human FBXW7 / FBW7 Antibody (Internal, IHC, WB) LS‑B2909

Note: This antibody replaces LS-C14412
Antibody:
Rabbit Polyclonal to Human FBXW7 / FBW7
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B2909-50
50 µg
0.25 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human FBXW7 / FBW7
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FBW7 antibody LS-B2909 is an unconjugated rabbit polyclonal antibody to human FBW7 (FBXW7) (Internal). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FBXW7 / FBW7
Synonyms
FBXW7 | AGO | Archipelago homolog | Archipelago | CDC4 | F-box protein FBW7 | FBXW6 | F-box protein FBX30 | F-box protein SEL-10 | HCdc4 | Homolog of C elegans sel-10 | FBW6 | FBX30 | FBW7 | HAgo | SEL-10 | SEL10
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10).
Epitope
Internal
Specificity
Recognizes human Cdc4 protein at ~60-80kD. Multiple bands may represent various isoforms.
Usage
Immunohistochemistry: LS-B2909 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2909 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FBXW7 / FBW7
Q969H0 NM_018315 NP_060785.2

Validation

FBXW7 / FBW7 Antibody - Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ FBXW7 / FBW7 Antibody (Internal) for IHC, WB/Western, IP LS-B2909 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/8/2020