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IHCPlus™ FBXW7 / FBW7 Antibody Internal Polyclonal IHC,IP,WB LS‑B2909

Catalog Number Size Price
LS-B2909-50 50 µg (0.25 mg/ml) $485 
Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ FBXW7 / FBW7 Antibody Internal Polyclonal IHC,IP,WB LS‑B2909

Note: This antibody replaces LS-C14412
Antibody:
Rabbit Polyclonal to Human FBXW7 / FBW7
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B2909-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FBXW7 / FBW7
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FBW7 antibody LS-B2909 is an unconjugated rabbit polyclonal antibody to human FBW7 (FBXW7 ). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FBXW7 / FBW7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10).
Epitope
Internal
Specificity
Recognizes human Cdc4 protein at ~60-80kD. Multiple bands may represent various isoforms.
Usage
Immunohistochemistry: LS-B2909 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2909 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FBXW7 / FBW7
Q969H0 NM_018315 NP_060785.2

Validation

Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FBXW7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/18/2019