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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B8366-50 50 µl (1 mg/ml) $425 
ESPL1 / Separase Antibody - Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
ESPL1 / Separase Antibody - Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.
ESPL1 / Separase Antibody - Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using SEPARASE Antibody. The lane on the right is blocked with the synthesized peptide.
ESPL1 / Separase Antibody - Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
ESPL1 / Separase Antibody - Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.
ESPL1 / Separase Antibody - Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using SEPARASE Antibody. The lane on the right is blocked with the synthesized peptide.
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IHCPlus™ Polyclonal ESPL1 / Separase Antibody (aa767‑816, IHC, IF, WB) LS‑B8366

IHCPlus™ Polyclonal ESPL1 / Separase Antibody (aa767‑816, IHC, IF, WB) LS‑B8366

Note: This antibody replaces LS-C117578
Antibody:
Rabbit Polyclonal to Human ESPL1 / Separase
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8366-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ESPL1 / Separase
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Separase antibody LS-B8366 is an unconjugated rabbit polyclonal antibody to Separase (ESPL1 ) from human and mouse. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ESPL1 / Separase
Synonyms
ESPL1 | ESP1 | Extra spindle poles like 1 | KIAA0165 | Caspase-like protein ESPL1 | SEPA | Separase | Separin
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human SEPARASE. (AA Range: 767-816)
Epitope
aa767-816
Specificity
SEPARASE (Ab-801) Antibody detects endogenous levels of total SEPARASE protein.
Usage
Western Blot: Predicted MW 233 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESPL1 / Separase
Q14674 NM_012291 NP_036423.4

Validation

ESPL1 / Separase Antibody - Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

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Requested From: United States
Date Requested: 2/25/2020