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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ESPL1 / Separase

extra spindle pole bodies homolog 1 (S. cerevisiae)

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).

Gene Name: extra spindle pole bodies homolog 1 (S. cerevisiae)
Family/Subfamily: Protease , Cysteine C50
Synonyms: ESPL1, ESP1, Extra spindle poles like 1, KIAA0165, Caspase-like protein ESPL1, SEPA, Separase, Separin
Target Sequences: NM_012291 NP_036423.4 Q14674

Publications (3)

1
Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells. Rubinek T, Chesnokova V, Wolf I, Wawrowsky K, Vlotides G, Melmed S. American journal of physiology. Cell physiology. 2007 293:C1082-92. [PubMed:17626243]
2
Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells. Fujita T, Epperly MW, Zou H, Greenberger JS, Wan Y. Molecular biology of the cell. 2008 19:5446-55. [PubMed:18843049] [PMC:PMC2592668]
3
Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner. Kim HS, Jeon YK, Ha GH, Park HY, Kim YJ, Shin HJ, Lee CG, Chung DH, Lee CW. Cancer research. 2009 69:27-36. [PubMed:19117984]

Filtered By: None
Target
ESPL1 / Separase (71)
Reactivity
Human (69)
Mouse (11)
Rat (1)
Application
IHC (49)
IHC-P (9)
WB (28)
DB (10)
ELISA (44)
ICC (4)
IF (16)
IP (3)
Peptide-ELISA (2)
Host
rabbit (57)
mouse (13)
goat (1)
Isotype
IgG (49)
IgG1 (3)
IgG1,k (7)
IgG2a (2)
IgG2a,k (1)
Clonality
monoclonal mc (13)
polyclonal pc (58)
Clone
2329C1a (2)
6H6 (1)
XJ11-1B12 (6)
XJ11-4D7 (1)
Format
AP Conjugated (5)
APC Conjugated (5)
Azide-free (5)
Biotin Conjugated (7)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (6)
HRP Conjugated (6)
PE Conjugated (5)
Unconjugated (34)
Epitope
pSer801 (10)
pSer1126 (9)
pSer1176 (7)
pSer1501 (7)
pSer748 (7)
C-Terminus (6)
Internal (3)
740-820 aa (2)
Ala795 (2)
aa1866-1996 (2)
N-Terminus (1)
aa1425-1475 (1)
aa767-816 (1)
Publications
No (71)
Showing 1-25 of 71 results

ESPL1 / Separase Antibody (N-Terminus) IHC-plus™ LS-B13240
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $485.00
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

ESPL1 / Separase Antibody (aa767-816) IHC-plus™ LS-B8366
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.

ESPL1 / Separase Antibody (clone 6H6) IHC-plus™ LS-B6199
Mouse Monoclonal [clone 6H6] (IgG2a,k) to Human ESPL1 / Separase
Human
ELISA IHC IHC-P WB
Unconjugated
50µg 0.31mg/ml  $485.00
Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Western blot of ESPL1 expression in HeLa NE.
Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Western blot of ESPL1 expression in HeLa NE.

ESPL1 / Separase Antibody (phospho-Ser801) LS-C354138
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Separase (pS801) staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Separase (pS801) staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

ESPL1 / Separase Antibody (Internal) LS-C358749
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC IF IHC IHC-P WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of Separase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Separase staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Separase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Separase staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

ESPL1 / Separase Antibody (phospho-Ser801) LS-C199560
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.

ESPL1 / Separase Antibody (740-820 aa) LS-C381459
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Phospho-Separase (S801) antibody
Western blot of Phospho-Separase (S801) antibody

ESPL1 / Separase Antibody (740-820 aa) LS-C386024
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Separase antibody
Western blot of Separase antibody
Western blot of Separase antibody
Western blot of Separase antibody

ESPL1 / Separase Antibody (Internal) LS-C91293
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC IHC IP WB
Unconjugated
100µl 0.2mg/ml  $435.00

ESPL1 / Separase Antibody (Ala795) LS-C176573
Rabbit Polyclonal to Human ESPL1 / Separase
Mouse, Human
IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
Western blot of Separase (A795) pAb in extracts from 293 cells.
IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
Western blot of Separase (A795) pAb in extracts from 293 cells.

ESPL1 / Separase Antibody LS-C403139
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

ESPL1 / Separase Antibody (phospho-Ser1126) LS-C328630
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
DB ELISA IHC
Unconjugated
200µl  $530.00

ESPL1 / Separase Antibody (phospho-Ser1126, Azide-free, HRP) LS-C282624
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
DB ELISA IHC
HRP Conjugated, Azide-free
200µl  $680.00

ESPL1 / Separase Antibody (phospho-Ser1126, AP) LS-C282621
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
DB ELISA IHC
AP Conjugated
200µl  $680.00

ESPL1 / Separase Antibody (phospho-Ser1126, APC) LS-C282622
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
DB ELISA IHC
APC Conjugated
200µl  $680.00

ESPL1 / Separase Antibody (aa1866-1996, clone XJ11-4D7) LS-C2889
Mouse Monoclonal [clone XJ11-4D7] (IgG2a) to Human ESPL1 / Separase
Human
IF WB
Unconjugated
100µl  $335.00
Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using Separase antibody, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.
Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using Separase antibody, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.

ESPL1 / Separase Antibody (aa1866-1996, clone XJ11-1B12) LS-C2890
Mouse Monoclonal [clone XJ11-1B12] (IgG1,k) to Human ESPL1 / Separase
Human
IF WB
Unconjugated
100µl  $335.00
Separase detected in 5C6 cell lysates. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Separase detected in 5C6 cell lysates. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ESPL1 / Separase Antibody (phospho-Ser801) LS-C192193
Rabbit Polyclonal to Human ESPL1 / Separase
Mouse, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
Immunohistochemistry (IHC) analysis of p-Separase (S801) pAb in paraffin-embedded human tonsil tissue.
Western blot of p-PLC beta 3 (S1105) pAb in extracts from A431 cells.
Immunohistochemistry (IHC) analysis of p-Separase (S801) pAb in paraffin-embedded human tonsil tissue.
Western blot of p-PLC beta 3 (S1105) pAb in extracts from A431 cells.

ESPL1 / Separase Antibody (phospho-Ser801) LS-C328626
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA IHC
Unconjugated
200µl  $530.00

ESPL1 / Separase Antibody LS-C500874
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA IF
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunofluorescent analysis of HepG2 cells using ESPL1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using ESPL1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ESPL1 / Separase Antibody (phospho-Ser748) LS-C328625
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA IHC
Unconjugated
200µl  $530.00

ESPL1 / Separase Antibody (phospho-Ser1501) LS-C328628
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA IHC
Unconjugated
200µl  $530.00

ESPL1 / Separase Antibody (phospho-Ser1176) LS-C330649
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA IHC
Unconjugated
200µl  $530.00

ESPL1 / Separase Antibody (phospho-Ser1126) LS-C336094
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
IF WB
Unconjugated
50µl  $265.00; 100µl  $375.00; 200µl  $495.00
Immunofluorescence analysis of U2OS cells.
Immunofluorescence analysis of U2OS cells.

ESPL1 / Separase Antibody (phospho-Ser1126) LS-C96968
Rabbit Polyclonal to Human ESPL1 / Separase
Human
DB IHC IHC-P
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Dot blot of anti-hSeparase-S801 Phospho-specific antibody on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Dot blot of anti-hSeparase-S801 Phospho-specific antibody on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Showing 1-25 of 71 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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