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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ESPL1 / Separase

extra spindle pole bodies homolog 1 (S. cerevisiae)

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).

Gene Name: extra spindle pole bodies homolog 1 (S. cerevisiae)
Family/Subfamily: Protease , Cysteine C50
Synonyms: ESPL1, ESP1, Extra spindle poles like 1, KIAA0165, Caspase-like protein ESPL1, SEPA, Separase, Separin
Target Sequences: NM_012291 NP_036423.4 Q14674

Publications (3)

1
Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells. Rubinek T, Chesnokova V, Wolf I, Wawrowsky K, Vlotides G, Melmed S. American journal of physiology. Cell physiology. 2007 293:C1082-92. [PubMed:17626243]
2
Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells. Fujita T, Epperly MW, Zou H, Greenberger JS, Wan Y. Molecular biology of the cell. 2008 19:5446-55. [PubMed:18843049] [PMC:PMC2592668]
3
Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner. Kim HS, Jeon YK, Ha GH, Park HY, Kim YJ, Shin HJ, Lee CG, Chung DH, Lee CW. Cancer research. 2009 69:27-36. [PubMed:19117984]

Viewing 1-25 of 72 items
ESPL1 / Separase Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC, IHC, IHC-P, IP, WB
Unconjugated
50µl   1mg/ml   $485.00
ESPL1 / Separase Antibody - IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
ESPL1 / Separase Antibody - Western blot of Separase antibody
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
ESPL1 / Separase Antibody - Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
ESPL1 / Separase Antibody - Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Select
Mouse Monoclonal [clone 6H6] (IgG2a,k) to Human ESPL1 / Separase
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $495.00
ESPL1 / Separase Antibody - Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $265.00
ESPL1 / Separase Antibody - Immunohistochemical analysis of Separase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl   1mg/ml   $205.00; 100µl   1mg/ml   $245.00; 200µl   1mg/ml   $305.00
ESPL1 / Separase Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
ESPL1 / Separase Antibody - Western blot of Phospho-Separase (S801) antibody
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Human
ICC, IHC, IP, WB
Unconjugated
100µl   $435.00
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA, IF, IHC-P, WB
Unconjugated
20µl   1mg/ml   $205.00; 60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
ESPL1 / Separase Antibody - Immunohistochemistry of paraffin-embedded Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA, IHC, WB
Unconjugated
20µl   0.5mg/ml   $205.00; 60µl   0.5mg/ml   $235.00; 120µl   0.5mg/ml   $295.00; 200µl   0.5mg/ml   $395.00
ESPL1 / Separase Antibody
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA, IF, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
DB, ELISA, IHC
Unconjugated
200µl   $590.00
ESPL1 / Separase Antibody - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using Separase antibody, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.
Select
Mouse Monoclonal [clone XJ11-4D7] (IgG2a) to Human ESPL1 / Separase
Human
IF, WB
Unconjugated
100µl   $335.00
ESPL1 / Separase Antibody - Separase detected in 5C6 cell lysates. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone XJ11-1B12] (IgG1,k) to Human ESPL1 / Separase
Human
IF, WB
Unconjugated
100µl   $335.00
ESPL1 / Separase Antibody - Immunohistochemistry (IHC) analysis of p-Separase (S801) pAb in paraffin-embedded human tonsil tissue.
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl   $345.00
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA, IHC
Unconjugated
200µl   $530.00
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA, IHC
Unconjugated
200µl   $530.00
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA, IHC
Unconjugated
200µl   $530.00
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA, IHC
Unconjugated
200µl   $530.00
ESPL1 / Separase Antibody - Immunofluorescent analysis of HepG2 cells using ESPL1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human
ELISA, IF
Unconjugated
50µg   $285.00; 100µg   $385.00
ESPL1 / Separase Antibody - Immunofluorescence analysis of U2OS cells.
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
IF, WB
Unconjugated
50µl   $265.00; 100µl   $375.00; 200µl   $495.00
ESPL1 / Separase Antibody - Immunohistochemistry of paraffin-embedded Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Select
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Mouse, Human
ELISA, IHC
Unconjugated
20µl   1mg/ml   $205.00; 60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
ESPL1 / Separase Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Select
Rabbit Polyclonal to Human ESPL1 / Separase
Human
DB, IHC, IHC-P
Unconjugated
400µl   $325.00

Viewing 1-25 of 72 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).