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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ESPL1 / Separase Antibody LS‑C403139
Separase antibody LS-C403139 is an unconjugated rabbit polyclonal antibody to Separase (ESPL1) from human and mouse. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C403139-20
20 µl
$205
LS-C403139-60
60 µl
$235
LS-C403139-120
120 µl
$295
LS-C403139-200
200 µl
$395

Popular ESPL1 / Separase Products

IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8366 dilution 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6199 concentration 3 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

Separase antibody LS-C403139 is an unconjugated rabbit polyclonal antibody to Separase (ESPL1) from human and mouse. Validated for ELISA, IHC and WB.
About ESPL1 / Separase
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. Q14674 NM_012291 NP_036423.4

ESPL1 Antibody, ESP1 Antibody, Extra spindle poles like 1 Antibody, KIAA0165 Antibody, Caspase-like protein ESPL1 Antibody, SEPA Antibody, Separase Antibody, Separin Antibody


Specifications

Target
Human ESPL1 / Separase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:40)
  • Western blot (1:500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide of human ESPL1
Specificity
Human ESPL1 / Separase
Presentation
PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Immunohistochemistry

Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Western blot

Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.

Immunohistochemistry

Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Immunohistochemistry

Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Western blot

Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.

Immunohistochemistry

Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Immunohistochemistry

Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Western blot

Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.

Immunohistochemistry

Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Immunohistochemistry

Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40.

Western blot

Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of Raji cell, using ESPL1 Polyclonal Antibody at dilution of 1:500.

Requested From: United States
Date Requested: 9/23/2018

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