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| Catalog Number | Size | Price |
|---|---|---|
| LS-C129775-200 | 200 µg | $919 |
Monoclonal Mouse His Tag Antibody (IF, WB) LS‑C129775
Monoclonal Mouse His Tag Antibody (IF, WB) LS‑C129775
Antibody:
His Tag Mouse Monoclonal Antibody
Application:
IF, WB, IP
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Mouse Monoclonal Antibody
Application:
IF, WB, IP
Format:
Unconjugated, Unmodified
Specifications
Description
His Tag antibody LS-C129775 is an unconjugated mouse monoclonal antibody to His Tag. Validated for IF, IP and WB.
Host
Mouse
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
6x His synthetic peptide.
Specificity
Recognizes His-tagged tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells. Species cross-reactivity: human, mouse, rat, others.
Applications
- Immunofluorescence
- Western blot (1:1000 - 1:4000)
- Immunoprecipitation (1:200 - 1:400)
Usage
Suitable for use in Western Blot, Immunoprecipitation, and Immunofluorescence. Western Blot: 1:1000-1:4000. Immunoprecipitation: 1:200-1:400.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
Date Requested: 4/29/2024
