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| Catalog Number | Size | Price |
|---|---|---|
| LS-C67918-100 | 100 µl (1 mg/ml) | $801 |
Monoclonal Mouse His Tag Antibody (AP, WB) LS‑C67918
Monoclonal Mouse His Tag Antibody (AP, WB) LS‑C67918
Antibody:
His Tag Mouse Monoclonal (AP) Antibody
Application:
WB, ELISA
Format:
Alkaline Phosphatase, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Mouse Monoclonal (AP) Antibody
Application:
WB, ELISA
Format:
Alkaline Phosphatase, Unmodified
Specifications
Description
His Tag antibody LS-C67918 is an AP-conjugated mouse monoclonal antibody to His Tag. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG2a
Monoclonal
Conjugations
Alkaline Phosphatase
Purification
Affinity purified
Modifications
Unmodified
Immunogen
His-tag-fusion protein.
Specificity
Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Applications
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. ELISA: 1:10000-1:50000. Use 50-100 ng control antigen/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.09% Sodium Azide
Storage
Store at 4°C. Do not freeze. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
