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| Catalog Number | Size | Price |
|---|---|---|
| LS-C67919-100 | 100 µl (1 mg/ml) | $801 |
Monoclonal Mouse His Tag Antibody (HRP, IHC, WB) LS‑C67919
Monoclonal Mouse His Tag Antibody (HRP, IHC, WB) LS‑C67919
Antibody:
His Tag Mouse Monoclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Mouse Monoclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Specifications
Description
His Tag antibody LS-C67919 is an HRP-conjugated mouse monoclonal antibody to His Tag. Validated for ELISA, IHC and WB.
Host
Mouse
Clonality
IgG2a
Monoclonal
Conjugations
Horseradish Peroxidase
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
His-tag fusion protein.
Specificity
Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Applications
- IHC (1:200 - 1:2000)
- Western blot (1:10000)
- ELISA (1:10000 - 1:50000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Western Blot: 1:10000. ELISA: 1:10000-1:50000 using 50-100 ng control antigen/well. Immunohistochemistry: 1:200-1:2000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05% merthiolate
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
