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His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The tag serves as a vector for the induced expression and production of that protein. His-tags are useful for protein purification due to the strong ionic affinity of histidines to various metals used in chromatography columns that allow for a tagged protein to be selected for in high purity.

His Tag Target Details

Target Name: His Tag

Publications (4)

1
Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. Yi CE, Ba L, Zhang L, Ho DD, Chen Z. Journal of virology. 2005 79:11638-46. [PubMed:16140741] [PMC:PMC1212612] Related Antibodies: LS-C60077.
2
Allosteric gating of Son of sevenless activity by the histone domain. Yadav KK, Bar-Sagi D. Proceedings of the National Academy of Sciences of the United States of America. 2010 107:3436-40. [PubMed:20133694] [PMC:PMC2840464] Related Antibodies: LS-C157807.
3
The Staphylococcus aureus superantigen SElX is a bifunctional toxin that inhibits neutrophil function. Tuffs SW, James DBA, Bestebroer J, Richards AC, Goncheva MI, O'Shea M, Wee BA, Seo KS, Schlievert PM, Lengeling A, van Strijp JA, Torres VJ, Fitzgerald JR. PLoS pathogens. 2017 13:e1006461. (Flo; Human) [Full Text Article] [PubMed:28880920] [PMC:PMC5589267] Related Antibodies: LS-C57341.
4
Novel FGFR4-Targeting Single-Domain Antibodies for Multiple Targeted Therapies against Rhabdomyosarcoma. Nagjie Alijaj, Sandrine Moutel, Zelia L Gouveia, Maxim Gray, Maurizio Roveri, Dzhangar Dzhumashev, Florian Weber, Gianmarco Meier, Paola Luciani, Jochen K Rössler, Beat W Schäfer, Franck Perez, Michele Bernasconi. Cancers. 2020 November;12: [Full Text Article] [PubMed:33182650] [PMC:PMC7696840] Related Antibodies: LS-C57341.
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☰ Filters
Antibodies (174)
ELISA Kits (1)
Competitive EIA (1)
Primary (174)
His Tag (175)
Human (13)
All species (13)
Bacteria (3)
Mammal (3)
Synthetic (5)
IHC (19)
IHC-Fr (1)
WB (154)
Flo (11)
ELISA (110)
ICC (12)
IF (24)
IP (56)
rabbit (48)
mouse (94)
goat (13)
chicken (9)
hamster-armenian (1)
human (4)
rat (4)
sheep (1)
IgG (54)
IgG,k (2)
IgG1 (36)
IgG1,k (20)
IgG2,k (1)
IgG2a (5)
IgG2a,k (5)
IgG2b (5)
IgG2b,k (2)
IgG2c,k (2)
IgG3,k (2)
IgG4,k (1)
IgY (4)
monoclonal mc (91)
polyclonal pc (68)
recombinant monoclonal rmc (15)
11E12 (1)
11n83 (1)
25B6E11 (1)
2A8 (1)
2C5.1 (1)
33D10.D2.G8 (3)
3D5 (15)
3G5H8 (1)
4D11 (2)
4E6 (2)
5I8 (1)
6.00E+02 (1)
6AT18 (1)
6C4 (1)
6G2A9 (2)
AD1.1.10 (7)
AD1.1.10.2 (1)
HIS.H8 (2)
HIS.H8/EH158 (1)
His17 (1)
M1 (1)
Mix (1)
T113 (1)
96-Well Strip Plate (1)
Agarose Conjugated (1)
AP Conjugated (7)
APC (2)
APC Conjugated (6)
Azide-free (1)
Biotin Conjugated (14)
Cy3 Conjugated (1)
Cy7 Conjugated (2)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (11)
HiLyte Fluor 488 Conjugated (1)
HiLyte Fluor 555 Conjugated (1)
HiLyte Fluor 647 Conjugated (1)
HRP Conjugated (21)
PE Conjugated (8)
Preservative Free (3)
Unconjugated (97)
His tag (C-term) (15)
His epitope (2)
C-Terminus (1)
No (172)
Yes (3)
Cell Culture Supernatants (1)
Cell Lysates (1)
Plasma (1)
Serum (1)
Tissue Homogenates (1)
His Tag Antibody - Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.
Select
His Tag Rabbit Polyclonal Antibody
ELISA, IHC, WB
Unconjugated
100 µg/$375
His Tag Antibody - Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
His Tag Mouse anti-Synthetic Monoclonal (FITC) (AD1.1.10) Antibody
Synthetic
Flo, IF
FITC Conjugated
0.1 mg/$417
Select
His Tag Rabbit Monoclonal Antibody
Flo, ICC, IHC, IP, WB
Unconjugated
50 µg/$466
His Tag Antibody - Detection of 6-His tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein
Select
His Tag Goat Polyclonal Antibody
ELISA, ICC, IP, WB
Unconjugated
100 µg/$483; 1 mg/$2,605
Select
His Tag Mouse Monoclonal (HRP) Antibody
ELISA, IHC, WB
HRP Conjugated
100 µl/$680
His Tag Antibody - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15 ug per well. Primary antibody: 6xHIS Tag antibody at 100 ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000. Substrate: TMB (p/n TMBE-1000). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
His Tag Mouse Monoclonal (Biotin) (33D10.D2.G8) Antibody
ELISA, IHC, WB
Biotin Conjugated
100 µg/$486
His Tag Antibody - Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
His Tag Mouse Monoclonal (HRP) (33D10.D2.G8) Antibody
ELISA, IHC, WB
HRP Conjugated
100 µg/$486
Select
His Tag Mouse anti-Synthetic Monoclonal (AD1.1.10) Antibody
Synthetic
ELISA, IP, WB
Unconjugated
0.1 mg/$313
His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
His Tag Rabbit Polyclonal (HRP) Antibody
ELISA, IHC, WB
HRP Conjugated
100 µg/$462
His Tag Antibody - Anti-6x-His Epitope Tag Monoclonal Antibody - Western Blot. Western blot of Immunochemicals Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.
Select
His Tag Mouse Monoclonal (33D10.D2.G8) Antibody
ELISA, IP, WB
Unconjugated
100 µg/$398
His Tag Antibody - Western blot of His-tagged fusion protein using His-tag Antibody, pAb, Rabbit (His-tag Antibody, pAb, Rabbit, 1 ug/ml) The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 30KD Observed Size: 30KD
Select
His Tag Rabbit Polyclonal (His epitope) Antibody
ELISA, WB
Unconjugated
40 µg/$285
Select
His Tag Sheep Polyclonal Antibody
IHC, WB
Unconjugated
500 µg/$369
Select
His Tag Mouse Monoclonal (AP) Antibody
ELISA, WB
AP Conjugated
100 µl/$680
His Tag Antibody - Western blot of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (THETM His Tag Antibody, mAb, Mouse, 1 ug/ml) Lane 1: N-terminal His-tagged fusion protein Lane 2: C-terminal His-tagged fusion protein The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit.
Select
His Tag Mouse Monoclonal (6G2A9) Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
100 µg/$519
His Tag Antibody - Western blot of lysates from 12tag protein , The His tagged antibody detected the His tagged protein (arrow).
Select
His Tag Mouse Monoclonal Antibody
WB
Unconjugated
200 µl/$391
Select
His Tag Mouse Monoclonal Antibody
ELISA, IF, IP, WB
Unconjugated
50 µl/$424
His Tag ELISA Kit
Select
Competitive EIA
96-Well Strip Plate
All species
24.69 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$898
Select
His Tag Mouse Monoclonal Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
100 µg/$801
Select
His Tag Goat Polyclonal Antibody
ELISA, IF, IHC, WB
Unconjugated
100 µg/$779
His Tag Antibody - Anti-6X His Epitope Tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
His Tag Rabbit Polyclonal (Biotin) Antibody
ELISA, IHC, WB
Biotin Conjugated
100 µg/$462
His Tag Antibody - Detection of 6-His tagged protein in 200, 200, and 50ng of E. coli lysate containing tagged fusion protein with 6-His Affinity-Purified Polyclonal Antibody, HRP Conjugate diluted 1:5000.
Select
His Tag Goat Polyclonal (HRP) Antibody
ELISA, ICC, WB
HRP Conjugated
100 µg/$547
Select
His Tag Rabbit Polyclonal Antibody
ELISA, IF, IHC, IP, WB
Unconjugated
100 µg/$325
His Tag Antibody
Select
His Tag Mouse Monoclonal (HIS.H8) Antibody
IF, IP, WB
Unconjugated
100 µg/$294
Select
His Tag Rabbit Polyclonal Antibody
ELISA, WB
Unconjugated
100 µl/$317
His Tag Antibody - Lane 1: Marker Lane 2: 10 ng Multiple Tag Cell Lysate fusion protein Lane 3: 1 ng Multiple Tag Cell Lysate fusion protein Lane 4: Negative control Detect antibody: 1 ug/ml THETM Anti-His [HRP] Monoclonal Antibody THETM His Tag Antibody [HRP], mAb, Mouse This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
His Tag Mouse Monoclonal (HRP) (6G2A9) Antibody
ELISA, WB
HRP Conjugated
100 µg/$549
Viewing 1-25 of 175 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).