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His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

His Tag Target Details

Target Name: His Tag

Publications (3)

1
Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. Yi CE, Ba L, Zhang L, Ho DD, Chen Z. Journal of virology. 2005 79:11638-46. [PubMed:16140741] [PMC:PMC1212612] Related Antibodies: LS-C60077.
2
Allosteric gating of Son of sevenless activity by the histone domain. Yadav KK, Bar-Sagi D. Proceedings of the National Academy of Sciences of the United States of America. 2010 107:3436-40. [PubMed:20133694] [PMC:PMC2840464] Related Antibodies: LS-C157807.
3
The Staphylococcus aureus superantigen SElX is a bifunctional toxin that inhibits neutrophil function. Tuffs SW, James DBA, Bestebroer J, Richards AC, Goncheva MI, O'Shea M, Wee BA, Seo KS, Schlievert PM, Lengeling A, van Strijp JA, Torres VJ, Fitzgerald JR. PLoS pathogens. 2017 13:e1006461. (Flo; Human) [Full Text Article] [PubMed:28880920] [PMC:PMC5589267] Related Antibodies: LS-C57341.

Filtered By: None
Target
his-tag (194)
Reactivity
Human (25)
All species (27)
Rabbit (1)
Application
IHC (33)
IHC-Fr (5)
WB (158)
Flo (12)
Block (1)
ELISA (104)
ICC (17)
IF (27)
IP (63)
Host
rabbit (60)
mouse (111)
goat (14)
chicken (8)
sheep (1)
Isotype
IgG (53)
IgG,k (1)
IgG1 (40)
IgG1,k (13)
IgG2a (5)
IgG2a,k (17)
IgG2b (7)
IgG2b,k (1)
IgY (5)
Clonality
monoclonal mc (113)
polyclonal pc (81)
Clone
11E12 (1)
11n83 (1)
25B6E11 (1)
2A8 (1)
2C5.1 (1)
33D10.D2 (2)
33D10.D2.G8 (1)
3G5H8 (1)
4D11 (2)
4E6 (2)
6C4 (1)
AD1.1.10 (8)
HIS.H8 (3)
His17 (1)
M1 (1)
Mix (1)
SP35 (1)
Format
Agarose Conjugated (1)
AMCA Conjugated (1)
AP Conjugated (10)
APC (3)
APC Conjugated (9)
Azide-free (1)
Biotin Conjugated (22)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FAM Conjugated (1)
FITC Conjugated (17)
HiLyte Fluor 488 Conjugated (3)
HiLyte Fluor 555 Conjugated (3)
HiLyte Fluor 594 Conjugated (1)
HiLyte Fluor 647 Conjugated (3)
HRP Conjugated (20)
PE Conjugated (10)
ROX Conjugated (1)
TAMRA Conjugated (1)
Unconjugated (82)
Epitope
C-Terminus (3)
aa6X His synthetic peptide conjugated to KLH. (1)
Publications
No (191)
Yes (3)
Showing 1-25 out of 194 results
Anti-His Tag Antibody (clone AD1.1.10, FITC) LS-C57341
Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Monoclonal [clone AD1.1.10] (IgG1)
Flo IHC
FITC Conjugated
Anti-His Tag Antibody LS-C60077
Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.

Rabbit Polyclonal
ELISA WB
Unconjugated
Anti-His Tag Antibody (clone HIS.H8) LS-C47232
Immunohistochemistry Image

Mouse Monoclonal [clone HIS.H8] (IgG2b)
IF IHC IP WB
Unconjugated
Anti-His Tag Antibody (HRP) LS-C67919

Mouse Monoclonal (IgG2a)
ELISA IHC WB
HRP Conjugated
Anti-His Tag Antibody (clone 33D10.D2, HRP) LS-C154015
Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Monoclonal [clone 33D10.D2] (IgG)
ELISA IHC WB
HRP Conjugated
Anti-His Tag Antibody (HRP) LS-C154274
Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Rabbit Polyclonal (IgG)
ELISA IHC WB
HRP Conjugated
Anti-His Tag Antibody (C-Terminus) LS-C51077
Western blot of His-tagged fusion protein using His-tag Antibody, pAb, Rabbit (LS-C51077, 1 ug/ml) The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 30KD Observed Size: 30KD
Western blot of His-tagged fusion proteins using His-tag Antibody, pAb, Rabbit (LS-C51077, 1 ug/ml) The signal was developed with One-Step Western Basic Kit. Predicted Size: Lane 1: His-tag fusion protein 52 kD Lane 2: C-term His-tag fusion protein 91 kD Observed Size: Lane 1: His-tag fusion protein 52 kD Lane 2: C-term His-tag fusion protein 91 kD

Rabbit Polyclonal (IgG)
ELISA WB
Unconjugated
Anti-His Tag Antibody LS-C33339

Mouse Monoclonal
ELISA WB
Unconjugated
Anti-His Tag Antibody (AP) LS-C67918

Mouse Monoclonal (IgG2a)
ELISA WB
AP Conjugated
Anti-His Tag Antibody (HiLyte Fluor 647) LS-C47733

Rabbit Polyclonal
HiLyte Fluor 647 Conjugated
Anti-His Tag Antibody (clone AD1.1.10, AP) LS-C188121

Mouse Monoclonal [clone AD1.1.10] (IgG1)
ELISA WB
AP Conjugated
Anti-His Tag Antibody LS-C157807
Western blot of lysates from 12tag protein , The His tagged antibody detected the His tagged protein (arrow).
HIS-tagged recombinant protein was expressed in E. coli, and used to test the 6xHIS monoclonal antibody (6AT18).

Mouse Monoclonal (IgG1)
WB
Unconjugated
Anti-His Tag Antibody LS-C136214

Sheep Polyclonal (IgG)
IHC WB
Unconjugated
Anti-His Tag Antibody (Biotin) LS-C51495
Western blot of His tagged fusion protein using THETM His Tag Antibody [Biotin], mAb, mouse (LS-C51495, 1 ug/ml) The signal was developed with Streptavidin-HRP and and LumiSensor HRP Substrate Kit Predicted Size: 52 KD Observed Size: 52 KD This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Monoclonal (IgG1)
ELISA WB
Biotin Conjugated
Anti-His Tag Antibody LS-C123755

Goat Polyclonal (IgG)
ELISA IF IHC WB
Unconjugated
Anti-His Tag Antibody (HiLyte Fluor 555) LS-C48105

Mouse Monoclonal
WB
HiLyte Fluor 555 Conjugated
Anti-His Tag Antibody LS-C83237

Goat Polyclonal
ELISA ICC IHC IP WB
Unconjugated
Anti-His Tag Antibody (clone 33D10.D2, Biotin) LS-C154017
ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15 ug per well. Primary antibody: 6xHIS Tag antibody at 100 ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000. Substrate: TMB (p/n TMBE-1000). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Monoclonal [clone 33D10.D2] (IgG)
ELISA IHC WB
Biotin Conjugated
Anti-His Tag Antibody LS-C137154

Rabbit Polyclonal (IgG)
ELISA WB
Unconjugated
Anti-His Tag Antibody LS-C51081
Western blot of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (LS-C51081, 1 ug/ml) Lane 1: N-terminal His-tagged fusion protein Lane 2: C-terminal His-tagged fusion protein The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit.
Western blot of Multiple Tag Cell Lysate using THETM His Antibody, mAb, Mouse (LS-C51081, 1 ug/ml) The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit. Predicted Size: 52 kD Observed Size: 52 kD

Mouse Monoclonal (IgG1)
ELISA Flo IF IP WB
Unconjugated
Anti-His Tag Antibody (TAMRA) LS-C48110

Mouse Monoclonal
IP WB
TAMRA Conjugated
Anti-His Tag Antibody LS-C67894

Mouse Monoclonal (IgG1,k)
ELISA Flo IF IP WB
Unconjugated
Anti-His Tag Antibody LS-C178368

Rabbit Polyclonal
ELISA IF IHC IP WB
Unconjugated
Anti-His Tag Antibody LS-C658638

Rabbit Monoclonal (IgG)
Flo ICC IHC IP WB
Unconjugated
Anti-His Tag Antibody (clone 25B6E11) LS-C742118

Rabbit Monoclonal [clone 25B6E11] (IgG) to Rabbit His Tag
Rabbit
ELISA Flo IF IP WB
Unconjugated

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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