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Catalog Number Size Price
LS-C153932-100 100 µg (1 mg/ml) $268 
Rabbit IgG Antibody - Western blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 25 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:40000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C153932

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C153932

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$268
LS-C153932-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C153932 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross-reactivity against human immunoglobulins.
Applications
  • IHC (1:500 - 1:5000)
  • Western blot (1:5000 - 1:40000)
  • ELISA (1:10000 - 1:100000)
  • Dot Blot
  • Electron Microscopy
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024