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Catalog Number Size Price
LS-C60887-2 2 mg (2 mg/ml) $321 
Rabbit IgG Antibody - HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated  (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60887

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60887

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$321
LS-C60887-2
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60887 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Applications
  • IHC (1:750 - 1:2500)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:50000 - 1:100000)
  • Dot Blot
  • Electron Microscopy
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022