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Goat Anti-Rabbit IgG Secondary Antibody (HRP) LS-C60887

Ordering

Wt. Vol. Conc. Price
2000 µg - 2 mg/ml $265
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Rabbit IgG Antibodies

Goat Anti-Rabbit IgG Secondary Antibody (PE) LS-C60868
Goat Polyclonal to Rabbit IgG
Rabbit
Immunofluorescence, Flow Cytometry
PE Conjugated
Goat Anti-Rabbit IgG Secondary Antibody (HRP) LS-C60884
Goat Polyclonal to Rabbit IgG
Rabbit
IHC, Western blot, ELISA
HRP Conjugated
Western blot Image
Goat Anti-Rabbit IgG Secondary Antibody (HRP) LS-C60887
Goat Polyclonal to Rabbit IgG
Rabbit
IHC, Western blot, ELISA
HRP Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Rabbit IgG
Rabbit
IHC, Western blot, ELISA
HRP Conjugated

Details

Rabbit IgG
Goat
Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Horseradish Peroxidase
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:750 - 1:2500)
  • Western blot (1:1500 - 1:5000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

Rabbit IgG antibody was raised against rabbit IgG whole molecule.
anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% Gentamicin sulfate.
Reconstitute with 1ml distilled water.
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.

About Rabbit IgG

IgG is the most common type of antibody found in the circulation, representing approximately 75% of serum antibodies. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape.

Western blot

Western blot
HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (LS-C60887 lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/6/2016

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