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Catalog Number Size Price
LS-C60921-1 1 mg (1 mg/ml) $383 
Rabbit IgG Antibody - Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Polyclonal Rat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60921

Polyclonal Rat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60921

Antibody:
Rabbit IgG Rat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$383
LS-C60921-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit IgG Rat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60921 is an HRP-conjugated rat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 1 publication.
Host
Rat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Goat, Human and Mouse Serum Proteins.
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:10000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

Rat Anti-Rabbit IgG Secondary Antibody (HRP) for IHC, WB/Western, ELISA LS-C60921 has an LSBio Rating of
Publications (4)

Publications (1)

Pleiotropic platelet defects in mice with disrupted FOG1-NuRD interaction. Wang Y, Meng R, Hayes V, Fuentes R, Yu X, Abrams CS, Heijnen HF, Blobel GA, Marks MS, Poncz M. Blood. Dec;118:6183-91.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022