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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C56309

Catalog Number Size Price
LS-C56309-2 2 mg $325 
Antibody

Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C56309

Antibody:
Goat Polyclonal to Rabbit Rabbit IgG
Application:
IHC-Fr, WB, ELISA
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$325 
LS-C56309-2
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Rabbit Rabbit IgG
Application:
IHC-Fr, WB, ELISA
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C56309 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 3 publications.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC - Frozen (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:25000)
  • ELISA (1:25000 - 1:125000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Rabbit IgG, whole molecule.
Specificity
Reacts with the heavy and light chains of rabbit IgG, as demonstrated by immunoelectrophoresis.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.01% Gentamicin Sulphate, 1% BSA
Reconstitution
Use 1 ml of sterile Distilled water.
Storage
Before reconstitution store at 4°C. After reconstitution store at 4°C or at -20°C. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (3)

Specific detection of OCT4 isoforms in inflammatory bowel disease. Maragkoudaki M, Vaiopoulou A, Theodoropoulos GE, Legaki E, Sechi LA, Karamanolis G, Zografos G, Gazouli M. Gut pathogens. 2015 7:25. (WB; Rabbit)

Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats. Papaconstantinou I, Zeglinas C, Gazouli M, Nastos K, Yiallourou A, Lykoudis P, Evangelou K, Papalois A, Papaioannou M, Vlachogiannakos J, Tzathas C. International journal of surgery (London, England). 2014 12:969-75. (WB; Rabbit)

Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. Sun M, Liu C, Zhao N, Meng K, Zhang Z. Experimental and therapeutic medicine. 2018 June;15:5215-5220. (Rabbit, Mouse)

Reviews (0)

Featured Products

Product
Species: Rabbit
Applications: Immunofluorescence, Flow Cytometry
RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
Product
Species: Rabbit
Applications: Immunofluorescence
HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated  (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated Western Blot. Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Rabbit IgG Load: 100 ng per lane Secondary antibody: RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
Reactivity: Rabbit
Range: 0.1-100 ng/ml

Requested From: United States
Date Requested: 9/17/2019