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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Donkey Anti‑Rabbit IgG Secondary Antibody (Fab'2, TR) LS‑C61494
Rabbit IgG antibody LS-C61494 is a TR-conjugated donkey polyclonal antibody. Validated for Flow and IF.
500 µg

Popular Rabbit IgG Products

Species: Rabbit
Applications: IHC, IHC - Frozen, Western blot, ELISA
Species: Rabbit
Applications: Immunofluorescence, Flow Cytometry
Species: Rabbit
Applications: Immunofluorescence
RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (LS-C60887 lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA

Product Description

Rabbit IgG antibody LS-C61494 is a TR-conjugated donkey polyclonal antibody. Validated for Flow and IF.
About Rabbit IgG
IgG is the most common type of antibody found in the circulation, representing approximately 75% of serum antibodies. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape.


Rabbit IgG
Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Texas Red
Immunoaffinity purified
  • Immunofluorescence (1:500 - 1:2500)
  • Flow Cytometry (1:2000 - 1:10000)
Rabbit IgG antibody was raised against rabbit IgG whole molecule.
anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA, 0.01% sodium azide.
Store at 4°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.

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Date Requested: 6/23/2018

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