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Catalog Number Size Price
LS-C343921-100 100 µg $397 
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody IHC-frozen: Human Placenta Tissue.
COL1A1 / Collagen I Alpha 1 Antibody - IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody. Collagen I/COL1A1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Collagen I/COL1A1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody IHC-paraffin: Rat Lung Tissue.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody IHC-paraffin: Human Intestinal Cancer Tissue.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody ICC: NIH3T3 Cell.
COL1A1 / Collagen I Alpha 1 Antibody - IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody Collagen I/COL1A1 was detected in paraffin-embedded section of human mammary tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Collagen I/COL1A1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
COL1A1 / Collagen I Alpha 1 Antibody - IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody Collagen I/COL1A1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Collagen I/COL1A1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody Western blot. Lane 1: Rat Lung Tissue Lysate. Lane 2: Human Placenta Tissue Lysate. Lane 3: Rat Testis Tissue Lysate.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody IHC-frozen: Human Placenta Tissue.
COL1A1 / Collagen I Alpha 1 Antibody - IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody. Collagen I/COL1A1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Collagen I/COL1A1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody IHC-paraffin: Rat Lung Tissue.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody IHC-paraffin: Human Intestinal Cancer Tissue.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody ICC: NIH3T3 Cell.
COL1A1 / Collagen I Alpha 1 Antibody - IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody Collagen I/COL1A1 was detected in paraffin-embedded section of human mammary tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Collagen I/COL1A1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
COL1A1 / Collagen I Alpha 1 Antibody - IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody Collagen I/COL1A1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Collagen I/COL1A1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
COL1A1 / Collagen I Alpha 1 Antibody - Collagen I antibody Western blot. Lane 1: Rat Lung Tissue Lysate. Lane 2: Human Placenta Tissue Lysate. Lane 3: Rat Testis Tissue Lysate.
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Polyclonal Rabbit anti‑Mouse COL1A1 / Collagen I Alpha 1 Antibody (aa1192‑1207, IHC, WB) LS‑C343921

Polyclonal Rabbit anti‑Mouse COL1A1 / Collagen I Alpha 1 Antibody (aa1192‑1207, IHC, WB) LS‑C343921

Note: This antibody replaces LS-C388236
Antibody:
COL1A1 / Collagen I Alpha 1 Rabbit anti-Mouse Polyclonal (aa1192-1207) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$397
LS-C343921-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
COL1A1 / Collagen I Alpha 1 Rabbit anti-Mouse Polyclonal (aa1192-1207) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen I Alpha 1 antibody LS-C343921 is an unconjugated rabbit polyclonal antibody to Collagen I Alpha 1 (COL1A1) (aa1192-1207) from mouse. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 1 publication.
Target
Mouse COL1A1 / Collagen I Alpha 1
Synonyms
COL1A1 | Alpha-1 type I collagen | Collagen alpha 1 chain type I | Collagen alpha-1(I) chain | Collagen, type I, alpha 1 | Collagen I | Pro-alpha-1 collagen type 1 | OI4
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of mouse Collagen I(1192-1207 aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.
Epitope
aa1192-1207
Specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen (0.5 - 1 µg/ml)
  • ICC (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL1A1 / Collagen I Alpha 1
P02452 NM_000088 NP_000079.2

LSBio Ratings

COL1A1 / Collagen I Alpha 1 Antibody (aa1192-1207) for IHC, ICC, WB/Western LS-C343921 has an LSBio Rating of
Publications (4)

Publications (1)

The Role of Periostin in Capsule Formation on Silicone Implants. Bae HS, Son HY, Lee JP, Chang H, Park JU. BioMed research international. 2018 April;2018:3167037. (Mouse, Human)

Customer Reviews (0)


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Requested From: United States
Date Requested: 6/15/2021