LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
BRAF / B‑Raf Antibody (aa171‑220) IHC‑plus™ LS‑B7672
Note: This antibody replaces LS-C118789
B-Raf antibody LS-B7672 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) from human and mouse. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7672-50
50 µl (1 mg/ml)
$425
Description
B-Raf antibody LS-B7672 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) from human and mouse. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BRAF / B-Raf
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BRAF / B-Raf antibody was raised against synthetic peptide from human B-RAF. (AA Range: 171-220)
Epitope
aa171-220
Specificity
B-RAF Antibody detects endogenous levels of total B-RAF protein.
Usage
Western Blot: Predicted MW 84 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Popular BRAF / B-Raf Products

Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Monkey, Dog, Horse
Applications: Western blot
Anti-BRAF antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
All lanes: Anti B Raf at 0.5 ug/ml. Lane 1: Rat Testis Tissue at 50 ug. Lane 2: Rat Brain Tissue at 50 ug. Lane 3: Mouse Testis Tissue at 50 ug. Lane 4: Mouse Brain Tissue at 50 ug. Lane 5: HeLa Whole Cell Lysate at 40 ug. Lane 6: Jurkat Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Lane 8: K562 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD . Observed band size: 84 kD.
Species: Human, Mouse, Rat
Applications: Western blot

Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence

Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot

Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry

Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence

Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot

Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry

Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence

Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot

Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry

Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence

Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot

Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.

Requested From: United States
Date Requested: 12/16/2018
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy