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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRAF / B-Raf

v-raf murine sarcoma viral oncogene homolog B

BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

Gene Name: v-raf murine sarcoma viral oncogene homolog B
Family/Subfamily: Protein Kinase , Raf
Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
Target Sequences: NM_004333 NP_004324.2 P15056

Filtered By: None
Target
BRAF / B-Raf (160)
Reactivity
Human (155)
Mouse (59)
Rat (44)
Bat (1)
Bovine (3)
Chicken (4)
Dog (6)
Horse (2)
Monkey (7)
Pig (1)
Sheep (1)
Zebrafish (1)
Application
IHC (89)
IHC-P (38)
WB (120)
Flo (7)
DB (32)
ELISA (78)
Func (1)
ICC (2)
IF (19)
IP (2)
Peptide-ELISA (9)
Host
rabbit (137)
mouse (22)
chicken (1)
Isotype
Ig (1)
IgG (109)
IgG1 (12)
IgG1,k (1)
IgG2a (4)
IgG2b (2)
IgY (1)
Clonality
monoclonal mc (23)
polyclonal pc (137)
Clone
1D2 (1)
1D4 (1)
1E2 (1)
1G6 (1)
1G8 (1)
1H12 (1)
1H12F1 (1)
1H12F1,1H12G10,1F12F11C9 (1)
3A7 (1)
3B12 (1)
3C3 (1)
3C6 (1)
3E8 (1)
4A5 (1)
4B2 (1)
4C11 (1)
4C5 (1)
4G6 (1)
5A9 (1)
Format
AP Conjugated (10)
APC Conjugated (10)
Azide-free (8)
Biotin Conjugated (11)
FITC Conjugated (11)
HRP Conjugated (11)
PE Conjugated (10)
Unconjugated (97)
Epitope
pSer445 (10)
Ser445 (8)
Ser601 (8)
Thr400 (8)
aa424-453 (8)
pThr400 (8)
pThr439 (8)
pThr598 (8)
pSer446 (4)
pThr599 (4)
380-460 aa (2)
540-620 aa (2)
550-630 aa (2)
700-780 aa (2)
aa10-22 (2)
aa25-75 (2)
pThr753 (2)
140-220 aa, Internal (1)
Ala597 (1)
C-Terminus (1)
Internal (1)
Lys439 (1)
Phe594 (1)
Tyr198 (1)
aa171-220 (1)
aa344-362 (1)
aa38-230 (1)
aa390-439 (1)
aa411-460 (1)
aa571-620 (1)
aa576-625 (1)
aa754-765 (1)
pSer602 (1)
pSer728 (1)
pThr598/Ser601 (1)
Publications
No (160)
Showing 1-25 of 160 results

BRAF / B-Raf Antibody (clone 1H12) IHC-plus™ LS-B3862
Mouse Monoclonal [clone 1H12] (IgG1) to Human BRAF / B-Raf
Human
ELISA IF IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

BRAF / B-Raf Antibody (phospho-Thr598/Ser601) IHC-plus™ LS-B1627
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Human
ICC IF IHC IHC-P
Unconjugated
50µl 0.5mg/ml  $425.00
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Thr598/Ser601 phosphorylated B-Raf, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.

BRAF / B-Raf Antibody (clone 1H12F1) IHC-plus™ LS-B3444
Mouse Monoclonal [clone 1H12F1] (IgG1) to Human BRAF / B-Raf
Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

BRAF / B-Raf Antibody (aa171-220) IHC-plus™ LS-B7672
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence analysis of HUVEC cells, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

BRAF / B-Raf Antibody IHC-plus™ LS-B10112
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Mouse, Rat, Human
ELISA IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-BRAF / B-Raf antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BRAF / B-Raf antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

BRAF / B-Raf Antibody (aa576-625) LS-C117802
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Mouse, Rat, Human
IHC Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.

BRAF / B-Raf Antibody (C-Terminus) LS-C353922
Rabbit Polyclonal to Human BRAF / B-Raf
Chicken, Zebrafish, Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of B-RAF staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of B-RAF expression in HL60 (A); HeLa (B); NIH3T3 (C) whole cell lysates.

BRAF / B-Raf Antibody LS-C658500
Rabbit Monoclonal (IgG) to Human BRAF / B-Raf
Human
IHC WB
Unconjugated
25µg  $395.00

BRAF / B-Raf Antibody (clone 1D4) LS-C114924
Mouse Monoclonal [clone 1D4] (IgG1) to Human BRAF / B-Raf
Human
Flo IF IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

BRAF / B-Raf Antibody (clone 1E2) LS-C114917
Mouse Monoclonal [clone 1E2] (IgG1) to Human BRAF / B-Raf
Flo IF IHC IHC-P WB
Unconjugated
100µl  $335.00
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

BRAF / B-Raf Antibody (clone 5A9) LS-C114534
Mouse Monoclonal [clone 5A9] (IgG1) to Human BRAF / B-Raf
Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BRAF mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody.

BRAF / B-Raf Antibody (aa10-22) IHC-plus™ LS-B12786
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Human
Func IHC IHC-P WB
Unconjugated
100µl  $485.00
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody (aa424-453) LS-C97187
Rabbit Polyclonal to Human BRAF / B-Raf
Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with BRAF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of C2C12 cell stained with BRAF Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). BRAF immunoreactivity is localized to Cytoplasm significantly.

BRAF / B-Raf Antibody (Ser445) LS-C97190
Rabbit Polyclonal to Human BRAF / B-Raf
Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (S445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of B-RAF Antibody (S445) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

BRAF / B-Raf Antibody (clone 3A7) LS-C114543
Mouse Monoclonal [clone 3A7] (IgG2a) to Human BRAF / B-Raf
Dog, Rat, Human, Monkey
IF IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

BRAF / B-Raf Antibody (Internal) LS-C351897
Rabbit Polyclonal to Human BRAF / B-Raf
Chicken, Mouse, Dog, Rat, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of B-RAF staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of B-RAF staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

BRAF / B-Raf Antibody (aa411-460) IHC-plus™ LS-B7587
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Mouse, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRAF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

BRAF / B-Raf Antibody (aa754-765) LS-C4218
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Dog, Horse, Human, Monkey
WB
Unconjugated
100µg 1mg/ml  $425.00

BRAF / B-Raf Antibody (clone 3E8) LS-C114544
Mouse Monoclonal [clone 3E8] (IgG2b) to Human BRAF / B-Raf
Human
IF IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded Human colon tissue using anti-BRAF mouse monoclonal antibody.
Anti-BRAF mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF.

BRAF / B-Raf Antibody (aa38-230) LS-C344029
Rabbit Polyclonal to Human BRAF / B-Raf
Mouse, Rat, Human
WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
All lanes: Anti B Raf at 0.5 ug/ml. WB: Recombinant Human B Raf Protein 0.5ng. Predicted band size: 40 kD. Observed band size: 40 kD.
All lanes: Anti B Raf at 0.5 ug/ml. Lane 1: Rat Testis Tissue at 50 ug. Lane 2: Rat Brain Tissue at 50 ug. Lane 3: Mouse Testis Tissue at 50 ug. Lane 4: Mouse Brain Tissue at 50 ug. Lane 5: HeLa Whole Cell Lysate at 40 ug. Lane 6: Jurkat Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Lane 8: K562 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD . Observed band size: 84 kD.

BRAF / B-Raf Antibody (140-220 aa, Internal) LS-C385754
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Raf-B antibody

BRAF / B-Raf Antibody (clone 1D2) LS-C114916
Mouse Monoclonal [clone 1D2] (IgG1) to Human BRAF / B-Raf
Human
Flo IF WB
Unconjugated
100µl  $335.00
Anti-BRAF mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

BRAF / B-Raf Antibody (aa25-75) LS-C288483
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Mouse, Dog, Bovine, Rat, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human BRAF by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRAF used at a dilution of 1:200 (1 ug/ml).

BRAF / B-Raf Antibody (Tyr198) LS-C176734
Rabbit Polyclonal to Human BRAF / B-Raf
Mouse, Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of Raf-B (Y198) pAb in paraffin-embedded human brain tissue.
Western blot of Raf-B (Y198) pAb in extracts from HeLa cells.

BRAF / B-Raf Antibody (clone 4A5) LS-C114532
Mouse Monoclonal [clone 4A5] (IgG2a) to Human BRAF / B-Raf
Human
IHC IHC-P WB
Unconjugated
100µl  $335.00
IHC of paraffin-embedded Kidney tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
Showing 1-25 of 160 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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