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Catalog Number Size Price
LS-B3862-50 50 µl $440 
BRAF / B-Raf Antibody - Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
BRAF / B-Raf Antibody - Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
BRAF / B-Raf Antibody - Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
BRAF / B-Raf Antibody - Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
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PathPlus™ Monoclonal Mouse anti‑Human BRAF / B‑Raf Antibody (clone 1H12, IHC, IF, WB) LS‑B3862

PathPlus™ Monoclonal Mouse anti‑Human BRAF / B‑Raf Antibody (clone 1H12, IHC, IF, WB) LS‑B3862

Note: This antibody replaces LS-C108748, LS-C758759, LS-C355318
Antibody:
BRAF / B-Raf Mouse anti-Human Monoclonal (1H12) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B3862-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BRAF / B-Raf Mouse anti-Human Monoclonal (1H12) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BRAF is a MAPK/ERK signaling pathway kinase involved in regulating growth, proliferation, apoptosis and survival of cells. It is one of the most frequently somatically mutated genes in cancer, particularly in melanomas, thyroid carcinomas, and colorectal cancer. BRAF mutations (most frequently the V600E mutation) occur in up to 60% of melanomas and more than 60% of papillary thyroid carcinomas. BRAF mutation is also present in roughly 10% of colorectal cancers, where mutation detection is useful for differentiating CIMP (CpG Island Methylator Phenotype, BRAF V600E positive) from Lynch syndrome (typically BRAF mutation negative). Status of BRAF mutation will predict response to therapy in cases of advanced melanoma. Furthermore, BRAF-mutant tumors tend to be more aggressive and are linked with shorter survival. Thus, determining presence of BRAF mutation is an important step in the differential diagnosis of numerous malignancies. Staining of wild-type BRAF may be nuclear, cytoplasmic or membranous and is present in all tissues. References: Nature Biotechnology. 2016. 34 (2):155–63, PMID: 26619011; Endocr Relat Cancer. 2014 Jan 30;21(2):161-73, PMID: 24243688; Mod Pathol. 2018 Jan; 31(1): 24–38, PMID: 29148538; Am J Transl Res. 2018; 10(8): 2726–2736, PMID: 30210710; Molecular Cancer Therapeutics. 2011 Mar. 10 (3):385, DOI: 10.1158/1535-7163.MCT-10-0799
Target
Human BRAF / B-Raf
Synonyms
BRAF | 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1H12
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of human BRAF expressed in E. Coli encoding aa 285-401.
Specificity
Human, Mouse
Applications
  • IHC - Paraffin (1:200 - 1:400)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Validation

BRAF / B-Raf Antibody - Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

LSBio Ratings

PathPlus™ BRAF / B-Raf Antibody (clone 1H12) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3862 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 5/21/2024