Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C4218-100 100 µg (1 mg/ml) $572 
Antibody

Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (aa754‑765, WB) LS‑C4218

Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (aa754‑765, WB) LS‑C4218

Antibody:
BRAF / B-Raf Rabbit anti-Human Polyclonal (aa754-765) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$572
LS-C4218-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BRAF / B-Raf Rabbit anti-Human Polyclonal (aa754-765) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
B-Raf antibody LS-C4218 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF) (aa754-765). Validated for WB.
Target
Human BRAF / B-Raf
Synonyms
BRAF | 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-Terminus cysteine added for conjugation to KLH.
Epitope
aa754-765
Specificity
Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). .
Applications
  • Western blot (1 - 2 µg/ml)
Usage
Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 ug.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/27/2024