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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BGN / Biglycan Antibody (aa215‑264) LS‑C111883
Biglycan antibody LS-C111883 is an unconjugated rabbit polyclonal antibody to Biglycan (BGN) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C111883-50
50 µg
$365

Popular BGN / Biglycan Products

Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Western blot
Western blot of recombinant BGN / Biglycan.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot

Product Description

Biglycan antibody LS-C111883 is an unconjugated rabbit polyclonal antibody to Biglycan (BGN) from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human BGN / Biglycan
Synonyms
BGN, Bone/cartilage proteoglycan I, Biglycan, Biglycan proteoglycan, DSPG1, PG-S1, Small leucine-rich protein 1A, PGI, Bone/cartilage proteoglycan-I, SLRR1A
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
BGN / Biglycan antibody was raised against synthetic peptide located between aa215-264 of human BGN (P21810, NP_001702). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Elephant (92%); Lizard (91%); Platypus (85%); Xenopus (83%).
Blocking Peptide
LS-E20430 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111883. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa215-264
Specificity
Human BGN / Biglycan
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BGN / Biglycan
BGN / Biglycan is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. P21810 NM_001711 NP_001702.1


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Images

Western blot

BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/12/2018

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