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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BGN / Biglycan Antibody (aa357‑368) LS‑C54578
Note: This antibody replaces LS-C76953
Biglycan antibody LS-C54578 is an unconjugated goat polyclonal antibody to Biglycan (BGN) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C54578-100
100 µg (0.5 mg/ml)
$405
Description
Biglycan antibody LS-C54578 is an unconjugated goat polyclonal antibody to Biglycan (BGN) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Target
Human BGN / Biglycan
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:8000) & (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Failed Applications
  • IHC - Paraffin
Immunogen
BGN / Biglycan antibody was raised against synthetic peptide C-DRLAIQFGNYKK from the C-terminus of human BGN / Biglycan (NP_001702.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Bovine, Dog, Horse, Pig, Platypus, Pufferfish, Zebrafish (100%); Panda, Lizard, Xenopus (92%); Stickleback (83%).
Blocking Peptide
LS-E26208 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54578. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa357-368
Specificity
Human BGN / Biglycan.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BGN / Biglycan
P21810 NM_001711 NP_001702.1

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Western blot of recombinant BGN / Biglycan.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Western blot

Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/16/2018
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