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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BGN / Biglycan Antibody (aa357-368) LS-C54578

Note: This antibody replaces LS-C76953
Antibody (0.1 ug/ml) staining of Rat Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BGN / Biglycan Antibody (aa357-368) LS-C54578

Note: This antibody replaces LS-C76953
Antibody:
Goat Polyclonal to Human BGN / Biglycan
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C54578-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human BGN / Biglycan
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
Biglycan antibody LS-C54578 is an unconjugated goat polyclonal antibody to Biglycan (BGN) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.

Target
Human BGN / Biglycan
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Immunogen
BGN / Biglycan antibody was raised against peptide with sequence C-DRLAIQFGNYKK, from the C Terminus of the protein sequence according to NP_001702.1.
Blocking Peptide
LS-E26208 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54578. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa357-368
Specificity
Human BGN / Biglycan.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BGN / Biglycan
P21810 NM_001711 NP_001702.1

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: Western blot
Reactivity: Human
Range: 13.7-10000 pg/ml
Reactivity: Rat
Range: 0.188-12 ng/ml
Renal tissue presenting zygomycosis stained with Mouse anti-Rhizopus Arrhizus
Species: Fungus
Applications: IHC - Paraffin, Western blot, ELISA

Requested From: United States
Date Requested: 6/26/2019
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