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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BGN / Biglycan Antibody (clone 4E1‑1G7) LS‑C196749 Best Seller
Biglycan antibody LS-C196749 is an unconjugated mouse monoclonal antibody to human Biglycan (BGN). Validated for ELISA, IHC, IP and WB.
Catalog
Size
Price
LS-C196749-100
100 µg (0.45 mg/ml)
$355
Description
Biglycan antibody LS-C196749 is an unconjugated mouse monoclonal antibody to human Biglycan (BGN). Validated for ELISA, IHC, IP and WB.
Target
Human BGN / Biglycan
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [4E1-1G7]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1 - 10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BGN / Biglycan antibody was raised against bGN (AAH02416.1, aa1-368 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human BGN / Biglycan
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About BGN / Biglycan
P21810 NM_001711 NP_001702.1

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Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
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Western blot of recombinant BGN / Biglycan.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BGN / Biglycan antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Immunohistochemistry

Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]
Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]

Western blot

BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.
BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.

Western blot

Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.
Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.

Immunohistochemistry

Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]
Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]

Western blot

BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.
BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.

Western blot

Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.
Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.

Immunohistochemistry

Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]
Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]

Western blot

BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.
BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.

Western blot

Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.
Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.

Immunohistochemistry

Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]
Immunohistochemistry of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 ug/ml]

Western blot

BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.
BGN monoclonal antibody (M01), clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.

Western blot

Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.
Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/11/2018
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