Bcl-2 antibody LS-B1173 is an unconjugated rabbit polyclonal antibody to human Bcl-2 (BCL2). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human BCL2 / Bcl-2
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (20 µg/ml)
- Western blot
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BCL2 / Bcl-2 antibody was raised against synthetic peptide from human BCL2 / Bcl-2.
Amino acids surrounding Threonine 56 of human BCL2
Immunohistochemistry: LS-B1173 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1173 was determined to be 20 ug/ml.
PBS, 0.02% Sodium Azide, 50% Glycerol
Short term: -20°C; Long term: -70°C.
For research use only.