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Catalog Number Size Price
LS-C357722-20 20 µg (0.2 mg/ml) $347 
LS-C357722-100 100 µg (0.2 mg/ml) $522 
BCL2 / Bcl-2 Antibody - Bcl-2 antibody immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
BCL2 / Bcl-2 Antibody - Bcl-2 antibody western blot.  This image was taken for the unmodified form of this product. Other forms have not been tested.
BCL2 / Bcl-2 Antibody - Bcl-2 antibody flow cytometry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
BCL2 / Bcl-2 Antibody - Bcl-2 antibody immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
BCL2 / Bcl-2 Antibody - Bcl-2 antibody western blot.  This image was taken for the unmodified form of this product. Other forms have not been tested.
BCL2 / Bcl-2 Antibody - Bcl-2 antibody flow cytometry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone 100/D5, IHC, IF, WB) LS‑C357722

Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone 100/D5, IHC, IF, WB) LS‑C357722

Antibody:
BCL2 / Bcl-2 Mouse anti-Human Monoclonal (100/D5) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$347
LS-C357722-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
BCL2 / Bcl-2 Mouse anti-Human Monoclonal (100/D5) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Bcl-2 antibody LS-C357722 is an unconjugated mouse monoclonal antibody to human Bcl-2 (BCL2). Validated for Flow, IF, IHC and WB.
Target
Human BCL2 / Bcl-2
Synonyms
BCL2 | Apoptosis regulator Bcl-2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
100/D5
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.
Immunogen
Amino acids G aa PAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%); Gorilla, Orangutan, Bovine, Goat (91%); Galago (82%).
Specificity
Human BCL2 / Bcl-2
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl-2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 and the small number in which the neoplastic cells are negative. ELISA: Order BSA-free format for coating.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCL2 / Bcl-2
P10415 NM_000633 NP_000624.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/5/2021