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Antibodies > BCL2 / Bcl-2 > LS-C675297
 
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Catalog Number Size Price
LS-C675297-50 50 µl (1 mg/ml) $316 
LS-C675297-100 100 µl $386 
BCL2 / Bcl-2 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
BCL2 / Bcl-2 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
BCL2 / Bcl-2 Antibody - Western Blot Positive WB detected in:HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All Lanes:BCL2 antibody at 1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa
BCL2 / Bcl-2 Antibody - Overlay histogram showing Jurkat cells stained with BCL2 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
BCL2 / Bcl-2 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
BCL2 / Bcl-2 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
BCL2 / Bcl-2 Antibody - Western Blot Positive WB detected in:HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All Lanes:BCL2 antibody at 1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa
BCL2 / Bcl-2 Antibody - Overlay histogram showing Jurkat cells stained with BCL2 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Monoclonal Rabbit anti‑Human BCL2 / Bcl‑2 Antibody (IHC, WB) LS‑C675297

Monoclonal Rabbit anti‑Human BCL2 / Bcl‑2 Antibody (IHC, WB) LS‑C675297

Submit Review LSBio Guarantee
Antibody:
BCL2 / Bcl-2 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675297-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BCL2 / Bcl-2 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Bcl-2 antibody LS-C675297 is an unconjugated rabbit monoclonal antibody to Bcl-2 (BCL2) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human BCL2 / Bcl-2
Synonyms
BCL2 | Apoptosis regulator Bcl-2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCL2 / Bcl-2
P10415 NM_000633 NP_000624.2

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Featured Products

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BCL2 / Bcl‑2 Antibody (clone E17, Concentrated) LS‑C202809
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
BCL2 / Bcl-2 Antibody - Anti-BCL2 / Bcl-2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ BCL2 / Bcl‑2 Antibody (aa46‑95) LS‑B6548
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
BCL2 / Bcl-2 Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
BCL2 / Bcl‑2 Antibody (aa41‑54, clone BL‑2, Magnetic beads) LS‑C171046
Species: Human
Applications: Immunoprecipitation
BCL2 / Bcl-2 Antibody - Formalin-fixed paraffin-embedded section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using Polyclonal Antibody to Bcl-2 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. A1 is a higher magnification of A.
BCL2 / Bcl‑2 Antibody (aa67‑86) LS‑C148229
Species: Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
BCL2 / Bcl-2 Antibody - Western blot of Bcl-2 using Polyclonal Antibody to Bcl-2 at 1:2000.20 ug/protein was loaded per lane. Lane 1. Human Jurkat T cells. Lane 2. Human RS11 lymphoma cells. Lanes 3-6. Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.
BCL2 / Bcl‑2 Antibody (aa41‑54) LS‑C148227
Species: Human, Monkey, Dog, Pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
BCL2 / Bcl-2 Antibody - Goat Anti-BCL2 Antibody (0.1µg/ml) staining of Human Lymph lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
BCL2 / Bcl‑2 Antibody (aa4‑18) LS‑C20131
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all BCL2 / Bcl-2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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