Reactivity
Yeast
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
Usage
This purified polyclonal antibody reacts yeast SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 12 kD band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee