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Yeast SUMO

Yeast Small Ubiquitin-related Modifier (SUMO / Smt3)


Yeast SUMO Target Details

Target Name: Yeast Small Ubiquitin-related Modifier (SUMO / Smt3)

Publications (1)

1
Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis. Vizeacoumar FJ, van Dyk N, S Vizeacoumar F, Cheung V, Li J, Sydorskyy Y, Case N, Li Z, Datti A, Nislow C, Raught B, Zhang Z, Frey B, Bloom K, Boone C, Andrews BJ. The Journal of cell biology. 2010 188:69-81. [PubMed:20065090] [PMC:PMC2812844] Related Antibodies: LS-C59540.

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Antibodies (5)
Primary (5)
Yeast SUMO (5)
Yeast (5)
IHC (1)
WB (5)
ELISA (5)
rabbit (3)
mouse (2)
IgG (2)
IgG1 (1)
IgG1,k (1)
monoclonal mc (2)
polyclonal pc (3)
4F2.F5.G2 (1)
Liquid (1)
Lyophilized (1)
Unconjugated (5)
aa1-97 (1)
No (4)
Yes (1)
Yeast SUMO Antibody - Anti-SUMO Antibody - Western Blot. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
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Yeast SUMO Antibody (Lyophilized) LS-C59540
(4.0) Rating
(1) Publication(s)
Yeast SUMO Rabbit anti-Yeast Polyclonal (Lyophilized) Antibody
Yeast
ELISA, WB
Unconjugated, Lyophilized
500 µg/$606
Yeast SUMO Antibody - Anti-ySUMO Antibody - Western Blot. Western blot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kD, the protein migrates as a 49 kD band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10000 dilution of IRDye 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results.
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Yeast SUMO Antibody (clone 4F2.F5.G2) LS-C59518
Yeast SUMO Mouse anti-Yeast Monoclonal (4F2.F5.G2) Antibody
Yeast
ELISA, IHC, WB
Unconjugated
100 µg/$574
Yeast SUMO Antibody - Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
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Yeast SUMO Antibody (Liquid) LS-C744971
Yeast SUMO Rabbit anti-Yeast Polyclonal (Liquid) Antibody
Yeast
ELISA, WB
Unconjugated, Liquid
25 µl/$304
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Yeast SUMO Antibody LS-C186427
Yeast SUMO Mouse anti-Yeast Monoclonal Antibody
Yeast
ELISA, WB
Unconjugated
500 µg/$431
Yeast SUMO Antibody - Western blot of SUMO antibody. Lane 1: SUMO adipotide (25 ng), Lane 2: SUMO hemokinin (25 ng)
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Yeast SUMO Antibody (aa1-97) LS-C204271
Yeast SUMO Rabbit anti-Yeast Polyclonal (aa1-97) Antibody
Yeast
ELISA, WB
Unconjugated
1 Each/$431
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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