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Antibodies > Yeast SUMO > LS-C59518
 
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Catalog Number Size Price
LS-C59518-100 100 µg (1.9 mg/ml) $574 
Yeast SUMO Antibody - Anti-ySUMO Antibody - Western Blot. Western blot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kD, the protein migrates as a 49 kD band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10000 dilution of IRDye 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results.

Monoclonal Mouse anti‑Yeast Yeast SUMO Antibody (clone 4F2.F5.G2, IHC, WB) LS‑C59518

Monoclonal Mouse anti‑Yeast Yeast SUMO Antibody (clone 4F2.F5.G2, IHC, WB) LS‑C59518

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Antibody:
Yeast SUMO Mouse anti-Yeast Monoclonal (4F2.F5.G2) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C59518-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Yeast SUMO Mouse anti-Yeast Monoclonal (4F2.F5.G2) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast SUMO antibody LS-C59518 is an unconjugated mouse monoclonal antibody to yeast Yeast SUMO. Validated for ELISA, IHC and WB.
Target
Yeast SUMO
Host
Mouse
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
4F2.F5.G2
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
This antibody was produced in mice by repeated immunizations with full-length recombinant yeast SUMO protein.
Applications
  • IHC (1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This monoclonal antibody reacts with yeast SUMO (Smt3) by western blot and ELISA. Using the specified conditions, this antibody may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Yeast SUMO Antibody - Anti-SUMO Antibody - Western Blot. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
Yeast SUMO Antibody (Lyophilized) LS‑C59540
Species: Yeast
Applications: Western blot, ELISA
Yeast SUMO Antibody - Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
Yeast SUMO Antibody (Liquid) LS‑C744971
Species: Yeast
Applications: Western blot, ELISA
IgE ELISA Kit
Rat IgE (Sandwich ELISA) ELISA Kit - LS-F36501
Reactivity: Rat
Range: 0.156-10 ng/ml
Product
Human HMG1 / HMGB1 Protein (Recombinant 6His, C-terminus) - LS-G145709
Source: E. coli
Tag: 6His, C-terminus
Thyrotropin / TSH ELISA Kit
Rat Thyrotropin / TSH (Competitive EIA) ELISA Kit - LS-F5124
Reactivity: Rat
Range: 24-15000 pg/ml
Lipoarabinomannan ELISA Kit
Lipoarabinomannan (Competitive EIA) ELISA Kit - LS-F13032
Reactivity:
Range: 0.312-20 ng/ml
See all Yeast SUMO Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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