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Antibodies > Yeast SUMO > LS-C744971
 
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Catalog Number Size Price
LS-C744971-25 25 µl (1 mg/ml) $304 
Yeast SUMO Antibody - Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Yeast Yeast SUMO Antibody (Liquid, WB) LS‑C744971

Polyclonal Rabbit anti‑Yeast Yeast SUMO Antibody (Liquid, WB) LS‑C744971

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Antibody:
Yeast SUMO Rabbit anti-Yeast Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C744971-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Yeast SUMO Rabbit anti-Yeast Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Liquid

Specifications

Description
Yeast SUMO antibody LS-C744971 is an unconjugated rabbit polyclonal antibody to yeast Yeast SUMO. Validated for ELISA and WB.
Target
Yeast SUMO
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Featured Products

Yeast SUMO Antibody - Anti-ySUMO Antibody - Western Blot. Western blot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kD, the protein migrates as a 49 kD band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10000 dilution of IRDye 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results.
Yeast SUMO Antibody (clone 4F2.F5.G2) LS‑C59518
Species: Yeast
Applications: IHC, Western blot, ELISA
Yeast SUMO Antibody - Anti-SUMO Antibody - Western Blot. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
Yeast SUMO Antibody (Lyophilized) LS‑C59540
Species: Yeast
Applications: Western blot, ELISA
Product
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Species: Human
Applications: IHC, Western blot, ELISA
CST7 / Cystatin F ELISA Kit
Mouse CST7 / Cystatin F (Sandwich ELISA) ELISA Kit - LS-F53016
Reactivity: Mouse
Range: 0.156-10 ng/ml
ST14 / Matriptase ELISA Kit
Human ST14 / Matriptase (Sandwich ELISA) ELISA Kit - LS-F49987
Reactivity: Human
Range: 0.41-100 ng/ml
Copeptin ELISA Kit
Human Copeptin (Sandwich ELISA) ELISA Kit - LS-F55567
Reactivity: Human
Range: 7.8-500 pg/ml
See all Yeast SUMO Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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