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Catalog Number Size Price
LS-C744971-25 25 µl (1 mg/ml) $275 
Yeast SUMO Antibody - Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Yeast Yeast SUMO Antibody (Liquid, WB) LS‑C744971

Polyclonal Rabbit anti‑Yeast Yeast SUMO Antibody (Liquid, WB) LS‑C744971

Antibody:
Yeast SUMO Rabbit anti-Yeast Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C744971-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Yeast SUMO Rabbit anti-Yeast Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Liquid

Specifications

Description
Yeast SUMO antibody LS-C744971 is an unconjugated rabbit polyclonal antibody to yeast Yeast SUMO. Validated for ELISA and WB.
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021