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Anti-Yeast SUMO Antibody (clone 4F2.F5.G2) LS-C59518


Wt. Vol. Conc. Price
100 µg - 1.9 mg/ml Unavailable

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Mouse Monoclonal [clone 4F2.F5.G2] to Yeast SUMO
Western blot, ELISA


Yeast SUMO
Yeast (tested or 100% immunogen sequence identity)
Monoclonal [4F2.F5.G2]
Protein A purified


  • Western blot (1:2000)
  • ELISA (1:20000)

Specificity and Use

Yeast SUMO antibody was raised against full- length recombinant yeast SUMO protein.
This monoclonal antibody reacts with yeast SUMO (Smt3) by western blot and ELISA. Using the specified conditions, this antibody may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Yeast SUMO

Western blot

Western blot
Anti-ySUMO Antibody - Western Blot. Western blot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kD, the protein migrates as a 49 kD band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10000 dilution of IRDye 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results.

Requested From: 
Date Requested: 3/30/2017

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