(tested or 100% immunogen sequence identity)
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
This purified polyclonal antibody reacts yeast SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 12 kD band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitute in 500 µL of deionized water.
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.