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Catalog Number Size Price
LS-C59540-500 500 µg (1 mg/ml) $541 
Yeast SUMO Antibody - Anti-SUMO Antibody - Western Blot. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Yeast Yeast SUMO Antibody (Lyophilized, WB) LS‑C59540

Polyclonal Rabbit anti‑Yeast Yeast SUMO Antibody (Lyophilized, WB) LS‑C59540

Antibody:
Yeast SUMO Rabbit anti-Yeast Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$541
LS-C59540-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Yeast SUMO Rabbit anti-Yeast Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Lyophilized

Specifications

Description
Yeast SUMO antibody LS-C59540 is an unconjugated rabbit polyclonal antibody to yeast Yeast SUMO. Validated for ELISA and WB. Cited in 1 publication.
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Usage
This purified polyclonal antibody reacts yeast SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 12 kD band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

Yeast SUMO Antibody (Lyophilized) for WB/Western, ELISA LS-C59540 has an LSBio Rating of
Publications (4)

Publications (1)

Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis. Vizeacoumar FJ, van Dyk N, S Vizeacoumar F, Cheung V, Li J, Sydorskyy Y, Case N, Li Z, Datti A, Nislow C, Raught B, Zhang Z, Frey B, Bloom K, Boone C, Andrews BJ. The Journal of cell biology. 2010 188:69-81.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/15/2022