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Anti-Yeast Rad9 Antibody (phospho-Ser1129) LS-C60075

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Related Products

Most Popular Yeast Rad9 Antibodies

Anti-Yeast Rad9 Antibody (aa1249-1263) LS-C17553
Rabbit Polyclonal to Yeast Rad9
Yeast
ELISA
Unconjugated
Anti-Yeast Rad9 Antibody (aa1125-1139) LS-C17554
Rabbit Polyclonal to Yeast Rad9
Yeast
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Yeast Rad9
Yeast
Western blot, ELISA
Unconjugated

Details

Yeast Rad9
Rabbit
Yeast (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • ELISA (1:100 - 1:500)

Specificity and Use

Yeast Rad9 antibody was raised against synthetic peptide corresponding to aa 1125-1139 of 1309 of yeast Rad9 protein conjugated to KLH.
pSer1129
an internal sequence of yeast Rad9 at the S1129 residue. Antiserum was purified against the immunizing peptide. This pan reactive polyclonal antibody reacts equally with both phosphorylated and nonphosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown
This pan reactive polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of the peptide and protein. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown. Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation. This product has been assayed against 0.1 ug of immunizing peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) catalog no. TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5000 is suggested for this product. Reactivity was detected against both the phosphorylated and non-phosphorylated form (S1129 and pS1129) of the immunizing peptide. This antibody appears to be pan reactive for both forms of the protein. Dilute the antibody 1:100 to 1:500 for immunoblotting. Researchers should determine optimal titers for other applications.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About Yeast Rad9


Requested From: United States
Date Requested: 12/4/2016

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