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Yeast Rad9


Yeast Rad9 Target Details

Target Name: Yeast Rad9
Target Abbreviation: Rad9

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Products
Antibodies (6)
Primary (6)
Yeast Rad9 (6)
Saccharomyces cerevisiae (1)
Yeast (5)
WB (5)
ELISA (6)
rabbit (6)
IgG (4)
polyclonal pc (6)
Unconjugated (6)
aa1125-1139 (1)
aa1249-1263 (1)
pSer1129 (1)
pSer1260 (1)
No (6)
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Yeast Rad9 Antibody (phospho-Ser1129) LS-C60074
Yeast Rad9 Rabbit anti-Yeast Polyclonal (pSer1129) Antibody
Yeast
ELISA, WB
Unconjugated
100 µg/$567
Select
Yeast Rad9 Antibody LS-C60081
Yeast Rad9 Rabbit anti-Yeast Polyclonal Antibody
Yeast
ELISA, WB
Unconjugated
100 µg/$567
Select
Yeast Rad9 Antibody (aa1125-1139) LS-C17554
Yeast Rad9 Rabbit anti-Yeast Polyclonal (aa1125-1139) Antibody
Yeast
ELISA, WB
Unconjugated
100 µg/$973
Yeast Rad9 Antibody - Affinity purified antibody to yeast Rad9 (1pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Detection occurred using a 1:5,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Yeast Rad9 Antibody LS-C60075
Yeast Rad9 Rabbit anti-Yeast Polyclonal Antibody
Yeast
ELISA, WB
Unconjugated
100 µg/$567
Yeast Rad9 Antibody - Anti-Rad9 Antibody- Western Blot. Affinity purified phospho-specific antibody to yeast Rad9 at pS1260 was used at a 1:200 dilution incubated overnight at 4° C to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kD) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Rad53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110 kD bands (asterisk). Detection occurred using a 1:5000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Select
Yeast Rad9 Antibody (phospho-Ser1260) LS-C60082
Yeast Rad9 Rabbit anti-Saccharomyces cerevisiae Polyclonal (pSer1260) Antibody
Saccharomyces cerevisiae
ELISA, WB
Unconjugated
100 µg/$567
Select
Yeast Rad9 Antibody (aa1249-1263) LS-C17553
Yeast Rad9 Rabbit anti-Yeast Polyclonal (aa1249-1263) Antibody
Yeast
ELISA
Unconjugated
100 µg/$973
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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