Yeast Rad9

Rabbit

Yeast (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1120-1145 from the aa1309 yeast Rad9 protein conjugated to KLH.

pSer1129

This phosphor polyclonal antibody reacts with the phosphorylated Rad9 pS1129 and minimally with non-phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown.

• Western blot (1:500 - 1:2000)
• ELISA (1:10000 - 1:50000)

This phospho specific polyclonal antibody was tested by ELISA. Data from ELISA indicates the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. No reactivity is expected against the human or mouse analogs of RAD9. Reactivity against RAD9 from other sources is unknown. Although not tested, this antibody is likely functional by WB, IHC and IP. This product has been assayed against 0.1 ug of phosphorylated peptide (pS1129) in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:5000 is suggested for this product. Minimal reactivity was detected against the nonphosphorylated form of the immunizing peptide. This antibody appears to be specific for the active form (phosphorylated) of the protein. Researchers should determine optimal titers for other applications.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.