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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C60082-100 100 µg (0.41 mg/ml) $425 
Yeast Rad9 Antibody - Anti-Rad9 Antibody- Western Blot. Affinity purified phospho-specific antibody to yeast Rad9 at pS1260 was used at a 1:200 dilution incubated overnight at 4° C to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kD) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Rad53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110 kD bands (asterisk). Detection occurred using a 1:5000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Saccharomyces cerevisiae Yeast Rad9 Antibody (phospho‑Ser1260, WB) LS‑C60082

Polyclonal Rabbit anti‑Saccharomyces cerevisiae Yeast Rad9 Antibody (phospho‑Ser1260, WB) LS‑C60082

Note: This antibody replaces LS-C17556
Antibody:
Rabbit Polyclonal to Saccharomyces cerevisiae Yeast Rad9
Application:
WB, ELISA
Reactivity:
Saccharomyces cerevisiae
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60082-100
100 µg
0.41 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Saccharomyces cerevisiae Yeast Rad9
Application:
WB, ELISA
Reactivity:
Saccharomyces cerevisiae
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Rad9 antibody LS-C60082 is an unconjugated rabbit polyclonal antibody to saccharomyces cerevisiae Yeast Rad9 (pSer1260). Validated for ELISA and WB.
Target
Saccharomyces cerevisiae Yeast Rad9
Synonyms
Rad9
Host
Rabbit
Reactivity
Saccharomyces cerevisiae (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of an internal region near aa 1225-1275 from the aa1309 yeast Rad9 protein conjugated to KLH.
Epitope
pSer1260
Usage
This phospho specific polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against the human or mouse analogs of RAD9. Reactivity against RAD9 from other sources is unknown. Cross reactivity may occur with auto-phosphorylated Rad53 kinase. Although not tested, this antibody is likely functional by IHC and IP. This product has been assayed against 0.1 ug of phosphorylated peptide (pS1260) in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) catalog no. TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5000 is suggested for this product. Minimal reactivity was detected against the nonphosphorylated form of the immunizing peptide. This antibody appears to be specific for the active form (phosphorylated) of the protein. Dilute the antibody 1:100 to 1:500 for immunoblotting. Researchers should determine optimal titers for other applications.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 8/7/2020