Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Vimentin is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification.
Immunofluorescence: Vimentin Antibody (2D1) - View of mixed neuron/glial cultures stained with Vimentin antibody (green) and the GFAP rabbit polyclonal (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.
Western Blot: Vimentin Antibody (2D1) - Western blot of crude extract HeLa. Lane 18 was probed with Vimentin antibody. Note the strong clean band at the expected molecular weight of 50kDa. Lane 17 was probed with a monoclonal antibody to Vimentin. Lane 15 was probed with an antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and Lane 16 with an antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.
Requested From: United States
Date Requested: 2/24/2017