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Catalog Number Size Price
LS-B11893-50 50 µl (1 mg/ml) $460 
Vimentin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Vimentin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Vimentin Antibody - View of mixed neuron/glial cultures stained with vimentin antibody (green) and rabbit antibody to GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Vimentin Antibody - Western blot of crude extract of the human carcinoma HeLa cell line. Lane 18 was probed with Anti-Vimentin Antibody.
Vimentin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Vimentin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Vimentin Antibody - View of mixed neuron/glial cultures stained with vimentin antibody (green) and rabbit antibody to GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Vimentin Antibody - Western blot of crude extract of the human carcinoma HeLa cell line. Lane 18 was probed with Anti-Vimentin Antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human Vimentin Antibody (clone 2A52, IHC, IF, WB) LS‑B11893

IHC‑plus™ Monoclonal Mouse anti‑Human Vimentin Antibody (clone 2A52, IHC, IF, WB) LS‑B11893

Note: This antibody replaces LS-C204596
Antibody:
Vimentin Mouse anti-Human Monoclonal (2A52) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11893-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Vimentin Mouse anti-Human Monoclonal (2A52) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Vimentin antibody LS-B11893 is an unconjugated mouse monoclonal antibody to Vimentin from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Vimentin
Synonyms
VIM | Vimentin | vimenin
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
2A52
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.
Specificity
Clone 2A52 is known to react with vimentin from human, cow, pig, mouse, rat and other mammals. Since vimentin is highly conserved, it is likely that the antibody is effective on other species also.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Vimentin
P08670 NM_003380 NP_003371.2

Validation

Vimentin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Vimentin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ Vimentin Antibody (clone 2A52) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B11893 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/4/2024