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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPP1 / Osteopontin Antibody LS‑C9413
Osteopontin antibody LS-C9413 is an unconjugated rabbit polyclonal antibody to human Osteopontin (SPP1). Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C9413-100
100 µl
$475

Popular SPP1 / Osteopontin Products

Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2411 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B425 dilution 1:500.
Species: Human, Mouse, Rat, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
SPP1 / Osteopontin antibody LS-C31763 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig
Applications: IHC, IHC - Paraffin, Western blot
Western blot of SPP1 / Osteopontin antibody LS-X223134.
Species: Human
Applications: Western blot, ELISA

Product Description

SPP1 / Osteopontin Antibody for ELISA, IHC, WB/Western LS-C9413

Specifications

Target
Human SPP1 / Osteopontin
Synonyms
SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:250 - 1:1000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide C-KSKKFRRPDIQYPD corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (93%).
Specificity
Recognizes the full length Human osteopontin protein at 66kD, C-terminal fragments of both thrombin and MMP-cleaved OPN at 32kD. Does not recognizes the 40kD N-terminal fragment.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Immunohistochemistry (paraffin): 1:50-1:150. Western Blot: 1:250-1:1000.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, 40% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SPP1 / Osteopontin
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. P10451 NM_000582 NP_000573.1


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Requested From: United States
Date Requested: 10/16/2018

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