Human SPP1 / Osteopontin
(tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
- IHC - Paraffin
- Western blot (1:250 - 1:1000)
- ELISA (1:10000 - 1:50000)
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A synthetic peptide C-KSKKFRRPDIQYPD corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (93%).
Recognizes the full length Human osteopontin protein at 66kD, C-terminal fragments of both thrombin and MMP-cleaved OPN at 32kD. Does not recognizes the 40kD N-terminal fragment.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Immunohistochemistry (paraffin): 1:50-1:150. Western Blot: 1:250-1:1000.
PBS, pH 7.2, 0.1% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.