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Catalog Number Size Price
LS-C100769-200 200 µl $393 
SPHK / SPHK1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SPHK / SPHK1 Antibody - Western blot of anti-SPHK1 Antibody in 293 cell line lysates (35 ug/lane). SPHK1 (arrow) was detected using the purified antibody.
SPHK / SPHK1 Antibody - Western blot of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
SPHK / SPHK1 Antibody - Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SPHK / SPHK1 Antibody - The anti-SPHK1 antibody is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
SPHK / SPHK1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SPHK / SPHK1 Antibody - Western blot of anti-SPHK1 Antibody in 293 cell line lysates (35 ug/lane). SPHK1 (arrow) was detected using the purified antibody.
SPHK / SPHK1 Antibody - Western blot of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
SPHK / SPHK1 Antibody - Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SPHK / SPHK1 Antibody - The anti-SPHK1 antibody is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
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Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (aa286‑315, IHC, WB) LS‑C100769

Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (aa286‑315, IHC, WB) LS‑C100769

Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal (aa286-315) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C100769-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal (aa286-315) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
SPHK1 antibody LS-C100769 is an unconjugated rabbit polyclonal antibody to SPHK1 (SPHK) (aa286-315) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
Target
Human SPHK / SPHK1
Synonyms
SPHK1 | SK1 | Sphingosine kinase 1 | SPHK | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Host
Rabbit
Reactivity
Human, Mouse, Rat, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa286-315
Specificity
This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPHK / SPHK1
Q9NYA1 NM_021972 NP_068807.2

LSBio Ratings

SPHK / SPHK1 Antibody (aa286-315) for IHC, WB/Western LS-C100769 has an LSBio Rating of
Publications (4)

Publications (1)

Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions. Fischer I, Alliod C, Martinier N, Newcombe J, Brana C, Pouly S. PloS one. 2011 6:e23905.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/26/2024