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Anti-SPHK / SPHK1 Antibody (aa286-315) LS-C100769

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SPHK / SPHK1 Antibodies

Anti-SPHK / SPHK1 Antibody (N-Terminus) IHC-plus™ LS-A3106
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-SPHK / SPHK1 Antibody (aa176-189) LS-C87246
Goat Polyclonal to Human SPHK / SPHK1
Human, Monkey, Mouse, Hamster
Western blot, ELISA
Unconjugated
Western blot Image
Anti-SPHK / SPHK1 Antibody (FITC) LS-C146698
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Human, Mouse
Western blot, Flow Cytometry
FITC Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Mouse, Rat, Primate
IHC - Paraffin, Western blot
Unconjugated

Details

Human SPHK / SPHK1
Rabbit
Human, Mouse, Rat, Primate (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

  • IHC - Paraffin

Specificity and Use

SPHK / SPHK1 antibody was raised against kLH-conjugated synthetic peptide between amino acids 286-315 from internal region of human SPHK1.
LS-E8787 - Lyophilized - 100 µg - $145.00
aa286-315

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions. Fischer I, Alliod C, Martinier N, Newcombe J, Brana C, Pouly S. PloS one. 2011 6:e23905. [PubMed:21887342] [PMC:PMC3161076]

About SPHK / SPHK1

Q9NYA1 NM_021972 NP_068807.2

SPHK1 Antibody, SK1 Antibody, Sphingosine kinase 1 Antibody, SPHK Antibody, SK 1 Antibody, SPK 1 Antibody, Sphingosine kinase type 1 Antibody, SPK Antibody

SPHK / SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
Western blot of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.

Western blot

Western blot
Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
The anti-SPHK1 antibody is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.

Western blot

Western blot
Western blot of anti-SPHK1 Antibody in 293 cell line lysates (35 ug/lane). SPHK1 (arrow) was detected using the purified antibody.
 

Requested From: United States
Date Requested: 12/6/2016

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