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Anti-POLR2A / RNA polymerase II Antibody (phospho-Thr4, DY488) LS-C180612

Ordering

Wt. Vol. Conc. Price
- 100 µl 1.1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POLR2A / RNA polymerase II Antibodies

Anti-POLR2A / RNA polymerase II Antibody (aa1585-1634) IHC-plus™ LS-B6990
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLR2A / RNA polymerase II Antibody (C-Terminus) LS-C287522
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Xenopus, Zebrafish
Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human POLR2A / RNA polymerase II
Human, Chicken
Western blot, Dot Blot (applications tested for the base form of this product only)
DY488 Conjugated

Details

Human POLR2A / RNA polymerase II
Rabbit
Human, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Affinity purified
Unmodified

Applications

  • Western blot (1:1000)
  • Dot Blot (1:1000)
  • (applications tested for the base form of this product only)

Specificity and Use

POLR2A / RNA polymerase II antibody was raised against a synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928].
pThr4
This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium borate
Store at 4°C in the dark.
For research use only.

About POLR2A / RNA polymerase II

P24928 NM_000937 NP_000928.1

POLR2A Antibody, HRPB220 Antibody, HsRPB1 Antibody, POLR2 Antibody, RpIILS Antibody, RPOL2 Antibody, RNA Polymerase II Antibody, RPO2 Antibody, POLRA Antibody, RNA polymerase II subunit B1 Antibody, RPB1 Antibody, RPBh1 Antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other.

Western blot

Western blot
RNA polymerase II [p Thr4] Antibody - Flag-tagged Rpb1 was IP''d with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot: RNA polymerase II [p Thr4] Antibody - WB analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

Dot Blot
Dot Blot: RNA polymerase II [p Thr4] Antibody - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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