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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLR2A / RNA polymerase II

polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

Gene Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Synonyms: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
Target Sequences: NM_000937 NP_000928.1 P24928

Publications (3)

1
DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro. Reid J, Svejstrup JQ. The Journal of biological chemistry. 2004 279:29875-8. (WB, IP; Yeast) [PubMed:15166235]
2
Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoter. Walsh HE, Shupnik MA. Molecular endocrinology (Baltimore, Md.). 2009 23:237-50. [PubMed:19095772] [PMC:PMC2646621]
3
Plasma membrane associated transcription of cytoplasmic DNA. Cheng J, Torkamani A, Peng Y, Jones TM, Lerner RA. Proceedings of the National Academy of Sciences of the United States of America. 2012 109:10827-31. (ICC, WB; Human) [PubMed:22711823] [PMC:PMC3390847]

Viewing 1-25 of 63 items
POLR2A / RNA polymerase II Antibody - Anti-POLR2A / RNA polymerase II antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
POLR2A / RNA polymerase II Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal to Human POLR2A / RNA polymerase II
Zebrafish, Rat, Mouse, Dog, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
50µl   1mg/ml   $425.00
POLR2A / RNA polymerase II Antibody - Western blot of Rpb1 antibody
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
POLR2A / RNA polymerase II Antibody -  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone 4H8] to Human POLR2A / RNA polymerase II
Drosophila, Mouse, Hamster, Plant, Human, Yeast
ELISA, IF, IP, WB
Unconjugated
100µl   1mg/ml   $335.00
Select
Mouse Monoclonal (IgG1) to Human POLR2A / RNA polymerase II
Xenopus, Zebrafish, Insect, Drosophila, Horse, Rabbit, Mouse, Dog, Bovine, Bat, Rat, Hamster, Human, Monkey
IHC, IHC-Fr, WB
Unconjugated
100µl   $620.00
POLR2A / RNA polymerase II Antibody - Immunofluorescence analysis of HepG2 cells.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
IF, WB
Unconjugated
50µl   $235.00; 100µl   $315.00; 200µl   $425.00
POLR2A / RNA polymerase II Antibody - Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug for WB), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Human
IP, WB
Unconjugated
100µl   1mg/ml   $395.00
POLR2A / RNA polymerase II Antibody - Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (Ab-1619) antibody.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µg   100µl   1µg/µl   $305.00
POLR2A / RNA polymerase II Antibody - P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (phospho-Ser1619) antibody.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µg   100µl   1µg/µl   $305.00
POLR2A / RNA polymerase II Antibody
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
POLR2A / RNA polymerase II Antibody - Western blot of Human Hela. POLR2A antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human POLR2A / RNA polymerase II
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
POLR2A / RNA polymerase II Antibody - Western blot testing of lambda phosphatase treated and untreated human MCF7 cell lysate with phospho-POLR2A antibody. Predicted molecular weight 200-220 kDa but commonly observed at 250-270 kDa.
Select
Rabbit Monoclonal [clone AH-16] (IgG) to Human POLR2A / RNA polymerase II
Human
WB
Unconjugated
100µg   $435.00
POLR2A / RNA polymerase II Antibody - Western blot analysis of MCF-7 cell lysates using RNA polymerase II CTD repeat Antibody (Phospho-Ser 2 ), pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 217 kD Observed Size: 217 kD
Select
Rabbit Monoclonal (IgG) to Human POLR2A / RNA polymerase II
WB
Unconjugated
40µg   80µl   0.5mg/ml   $245.00; 200µg   $455.00
POLR2A / RNA polymerase II Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
POLR2A / RNA polymerase II Antibody - Western blot of Phospho-Rpb1 (S1619) antibody
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
POLR2A / RNA polymerase II Antibody - IHC of POLR2A (P1613) pAb in paraffin-embedded human breast carcinoma tissue.
Select
Rabbit Polyclonal to Human POLR2A / RNA polymerase II
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl   $345.00
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Rat, Human, Monkey
ELISA, IF, IHC-P, WB
Unconjugated
20µl   1mg/ml   $205.00; 60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
POLR2A / RNA polymerase II Antibody - Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:500 ( 0.4 ug/ml). Detection: DAB.
Select
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Mouse, Human
IHC, IP, WB
Unconjugated
100µl   0.2mg/ml   $415.00
POLR2A / RNA polymerase II Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
Mouse Monoclonal [clone OTI1E5] (IgG1) to Human POLR2A / RNA polymerase II
Human
IHC, WB
Unconjugated
100µl   1mg/ml   $345.00
POLR2A / RNA polymerase II Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
Mouse Monoclonal [clone OTI1E5] (IgG1) to Human POLR2A / RNA polymerase II
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
POLR2A / RNA polymerase II Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
Mouse Monoclonal [clone OTI2B7] (IgG1) to Human POLR2A / RNA polymerase II
Human
IHC, WB
Unconjugated
100µl   1mg/ml   $345.00
POLR2A / RNA polymerase II Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
Mouse Monoclonal [clone OTI2B7] (IgG1) to Human POLR2A / RNA polymerase II
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
POLR2A / RNA polymerase II Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
Mouse Monoclonal [clone OTI3C8] (IgG1) to Human POLR2A / RNA polymerase II
Human
IHC, WB
Unconjugated
100µl   1mg/ml   $345.00
POLR2A / RNA polymerase II Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
Mouse Monoclonal [clone OTI3C8] (IgG1) to Human POLR2A / RNA polymerase II
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
POLR2A / RNA polymerase II Antibody - Immunohistochemistry - Anti-POLR2A antibody
Select
Rabbit Polyclonal to Human POLR2A / RNA polymerase II
Human
ELISA, IHC, IHC-P
Unconjugated
100µg   $305.00

Viewing 1-25 of 63 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).