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Antibodies > POLR2A / RNA polymerase II > LS-B14574
 
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Catalog Number Size Price
LS-B14574-50 50 µl (1 mg/ml) $460 
POLR2A / RNA polymerase II Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
POLR2A / RNA polymerase II Antibody - Immunofluorescent analysis of Rpb1 CTD staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
POLR2A / RNA polymerase II Antibody - Western blot analysis of Rpb1 CTD expression in MCF7 (A); MDAMB435 (B) whole cell lysates.
POLR2A / RNA polymerase II Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
POLR2A / RNA polymerase II Antibody - Immunofluorescent analysis of Rpb1 CTD staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
POLR2A / RNA polymerase II Antibody - Western blot analysis of Rpb1 CTD expression in MCF7 (A); MDAMB435 (B) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (C‑Terminus, IHC, IF, WB) LS‑B14574

IHC‑plus™ Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (C‑Terminus, IHC, IF, WB) LS‑B14574

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C413089
Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14574-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
RNA polymerase II antibody LS-B14574 is an unconjugated rabbit polyclonal antibody to RNA polymerase II (POLR2A) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A | HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Rpb1 CTD.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of Rpb1 CTD protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR2A / RNA polymerase II
P24928 NM_000937 NP_000928.1

Validation

POLR2A / RNA polymerase II Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ POLR2A / RNA polymerase II Antibody (C-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B14574 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

Submit a Publication for LS-B14574

Customer Reviews (0)

Submit a review for LS-B14574

Featured Products

POLR2A / RNA polymerase II Antibody - Anti-POLR2A / RNA polymerase II antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ POLR2A / RNA polymerase II Antibody (aa1585‑1634) LS‑B6990
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
POLR2A / RNA polymerase II Antibody - Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug for WB), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
POLR2A / RNA polymerase II Antibody (C‑Terminus) LS‑C287522
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
POLR2A / RNA polymerase II Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
POLR2A / RNA polymerase II Antibody (phospho‑Ser, clone 4H8) LS‑C49196
Species: Human, Mouse, Hamster, Drosophila, Yeast, Plant
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Product
POLR2A / RNA polymerase II Antibody LS‑C26239
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect
Applications: IHC, IHC - Frozen, Western blot
POLR2A / RNA polymerase II Antibody - All lanes: Anti-POLR2A Antibody (monoclonal) (M01AA) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 217 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
POLR2A / RNA polymerase II Antibody (clone 1357CT288.1.37) LS‑C757523
Species: Human
Applications: Western blot, ELISA
POLR2A / RNA polymerase II ELISA Kit
Mouse/Human/Rat POLR2A / RNA polymerase II (Cell-Based ELISA) ELISA Kit - LS-F2264
Reactivity: Human, Mouse, Rat
Range: Positive/Negative
See all POLR2A / RNA polymerase II Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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