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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLB / DNA Polymerase Beta Antibody LS‑C22842
Antibody
DNA Polymerase Beta antibody LS-C22842 is an unconjugated mouse monoclonal antibody to human DNA Polymerase Beta (POLB). Validated for IHC and WB.
Catalog
Size
Price
LS-C22842-500
500 µl
Unavailable

Popular POLB / DNA Polymerase Beta Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-POLB / DNA Polymerase Beta antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7021 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of Rabbit Anti-POL (DNA polymerase beta) Antibody (LS-C153861). Lane 1: LN428 FLAG POL (control). Lane 2: A172. Load: 35 ug per lane. Primary antibody: POL antibody at 1:2000 for overnight at 4C. Secondary antibody: IRDye800 goat anti-rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry of paraffin-embedded human esophageal tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

DNA Polymerase Beta antibody LS-C22842 is an unconjugated mouse monoclonal antibody to human DNA Polymerase Beta (POLB). Validated for IHC and WB.
About POLB / DNA Polymerase Beta
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. P06746 NM_002690 NP_002681.1

POLB Antibody, DNA pol beta Antibody, Mutant DNA polymerase beta Antibody, DNA polymerase beta Antibody, DNA polymerase beta subunit Antibody


Specifications

Target
Human POLB / DNA Polymerase Beta
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
DNA polymerase beta protein.
Specificity
Recognizes DNA Polymerase, beta. Species cross-reactivity: human.
Usage
Western Blot (Ab 2-4 ug/ml for 2 hrs at RT). Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30min at RT). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: HeLa cells. Testis.
Presentation
10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/22/2018

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