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Catalog Number Size Price
LS-C745350-25 25 µl (1.4 mg/ml) $275 
POLB / DNA Polymerase Beta Antibody - Western Blot of rabbit Anti-POLß (DNA polymerase beta) antibody. Lane 1: LN428 FLAG POLß (control). Lane 2: A172. Load: 35 µg per lane. Primary antibody: POLß antibody at 1:2000 for overnight at 4°C. Secondary antibody: goat anti-rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.
POLB / DNA Polymerase Beta Antibody - Western Blot of rabbit anti-POLB antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: Raji Lsyate Lane 7: Ramos Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: POLB antibody at 1:5,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 38 kDa for POLB.
POLB / DNA Polymerase Beta Antibody - Western Blot of rabbit Anti-POLß (DNA polymerase beta) antibody. Lane 1: LN428 FLAG POLß (control). Lane 2: A172. Load: 35 µg per lane. Primary antibody: POLß antibody at 1:2000 for overnight at 4°C. Secondary antibody: goat anti-rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.
POLB / DNA Polymerase Beta Antibody - Western Blot of rabbit anti-POLB antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: Raji Lsyate Lane 7: Ramos Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: POLB antibody at 1:5,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 38 kDa for POLB.
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Polyclonal Rabbit anti‑Human POLB / DNA Polymerase Beta Antibody (C‑Terminus, WB) LS‑C745350

Polyclonal Rabbit anti‑Human POLB / DNA Polymerase Beta Antibody (C‑Terminus, WB) LS‑C745350

Antibody:
POLB / DNA Polymerase Beta Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745350-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
POLB / DNA Polymerase Beta Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Polymerase Beta antibody LS-C745350 is an unconjugated rabbit polyclonal antibody to human DNA Polymerase Beta (POLB) (C-Terminus). Validated for ELISA and WB.
Target
Human POLB / DNA Polymerase Beta
Synonyms
POLB | DNA pol beta | Mutant DNA polymerase beta | DNA polymerase beta | DNA polymerase beta subunit
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Bovine, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.
Epitope
C-Terminus
Specificity
This antibody reacts with overexpressed and endogenous POLß protein. A BLAST analysis was used to suggest reactivity with POLß from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLß from other sources has not been determined.
Applications
  • Western blot (0.5 µg/ml)
  • ELISA (1:50000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLB / DNA Polymerase Beta
P06746 NM_002690 NP_002681.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021