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Antibodies > POLB / DNA Polymerase Beta > LS-C331633
 
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Catalog Number Size Price
LS-C331633-20 20 µl $287 
LS-C331633-50 50 µl $341 
LS-C331633-100 100 µl $431 
LS-C331633-200 200 µl $539 
POLB / DNA Polymerase Beta Antibody - Immunohistochemistry of paraffin-embedded human esophageal tissue.
POLB / DNA Polymerase Beta Antibody - Immunohistochemistry of paraffin-embedded rat lung tissue.
POLB / DNA Polymerase Beta Antibody - Immunofluorescence analysis of U2OS cells using POLB antibody. Blue: DAPI for nuclear staining.
POLB / DNA Polymerase Beta Antibody - Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
POLB / DNA Polymerase Beta Antibody - Western blot analysis of extracts of various cell lines, using POLB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
POLB / DNA Polymerase Beta Antibody - Immunohistochemistry of paraffin-embedded human esophageal tissue.
POLB / DNA Polymerase Beta Antibody - Immunohistochemistry of paraffin-embedded rat lung tissue.
POLB / DNA Polymerase Beta Antibody - Immunofluorescence analysis of U2OS cells using POLB antibody. Blue: DAPI for nuclear staining.
POLB / DNA Polymerase Beta Antibody - Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
POLB / DNA Polymerase Beta Antibody - Western blot analysis of extracts of various cell lines, using POLB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human POLB / DNA Polymerase Beta Antibody (IHC, IF, WB) LS‑C331633

Polyclonal Rabbit anti‑Human POLB / DNA Polymerase Beta Antibody (IHC, IF, WB) LS‑C331633

Submit Review LSBio Guarantee
Antibody:
POLB / DNA Polymerase Beta Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C331633-20
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
POLB / DNA Polymerase Beta Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Polymerase Beta antibody LS-C331633 is an unconjugated rabbit polyclonal antibody to DNA Polymerase Beta (POLB) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human POLB / DNA Polymerase Beta
Synonyms
POLB | DNA pol beta | Mutant DNA polymerase beta | DNA polymerase beta | DNA polymerase beta subunit
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1). MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
Specificity
Human POLB / DNA Polymerase Beta
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:200 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 38kDa, while the observed MW by Western blot was 37kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLB / DNA Polymerase Beta
P06746 NM_002690 NP_002681.1

Publications (0)

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Featured Products

POLB / DNA Polymerase Beta Antibody - Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
POLB / DNA Polymerase Beta Antibody (C‑Terminus) LS‑C352683
Species: Human, Mouse, Rat, Bovine, Chicken
Applications: IHC, IHC - Paraffin, Western blot
POLB / DNA Polymerase Beta Protein
Human POLB / DNA Polymerase Beta Protein (Recombinant His) - LS-G1408
Source: E. coli
Tag: His
FCGRT / FCRN Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ FCGRT / FCRN Antibody LS‑B15532
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
TSG101 ELISA Kit
Human TSG101 (Sandwich ELISA) ELISA Kit - LS-F8581
Reactivity: Human
Range: 0.312-20 ng/ml
HPN / TMPRSS1 / Hepsin ELISA Kit
Human HPN / TMPRSS1 / Hepsin (Sandwich ELISA) ELISA Kit - LS-F34865
Reactivity: Human
Range: 0.78-50 ng/ml
AKAP11 / KIAA0629 Antibody - Western Blot; Sample: Recombinant AKAP11, Human.
AKAP11 / KIAA0629 Antibody (aa159‑258) LS‑C374339
Species: Human
Applications: Western blot
See all POLB / DNA Polymerase Beta Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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