Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Bovine (tested or 100% immunogen sequence identity)
Specificity and Use
PARP1 antibody was raised against peptide corresponding to N-terminus of cleavage site (214/215) of human PARP.
Recognizes the 85kD fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. Recognizes human and bovine cells.
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Flow Cytometry: Use 10 ul label 1E6 cells to detect cleaved PAR. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, BSA, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR.