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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PARP1 Antibody (N‑Terminus, FITC) LS‑C17209
PARP1 antibody LS-C17209 is an FITC-conjugated rabbit polyclonal antibody to PARP1 from human and bovine. Validated for Flow, IHC and WB.
Antibody
Catalog
Size
Price
LS-C17209-100
100 tst
$605

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Product Description

PARP1 antibody LS-C17209 is an FITC-conjugated rabbit polyclonal antibody to PARP1 from human and bovine. Validated for Flow, IHC and WB.

Specifications

Target
Human PARP1
Synonyms
PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL
Host
Rabbit
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Fluorescein
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PARP1 antibody was raised against peptide corresponding to N-terminus of cleavage site (214/215) of human PARP.
Epitope
N-Terminus
Specificity
Recognizes the 85kD fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. Recognizes human and bovine cells.
Usage
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Flow Cytometry: Use 10 ul label 1E6 cells to detect cleaved PAR. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About PARP1
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. P09874 NM_001618 NP_001609.2


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Requested From: United States
Date Requested: 10/19/2018

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