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Catalog Number Size Price
LS-C408267-20 20 µl $235 
LS-C408267-50 50 µl $275 
LS-C408267-100 100 µl $355 
LS-C408267-200 200 µl $455 
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human mammary cancer using PARP1 antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer tissue.
PARP1 Antibody - Immunohistochemistry of paraffin-embedded mouse stomach tissue.
PARP1 Antibody - Immunofluorescence analysis of U2OS cells using PARP1 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
PARP1 Antibody - Western blot analysis of extracts of various cell lines.
PARP1 Antibody - Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PARP1 Antibody - Western blot analysis of extracts of various cell lines, using PARP1 antibodyat 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
PARP1 Antibody - Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human mammary cancer using PARP1 antibodyat dilution of 1:100 (40x lens).
PARP1 Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer tissue.
PARP1 Antibody - Immunohistochemistry of paraffin-embedded mouse stomach tissue.
PARP1 Antibody - Immunofluorescence analysis of U2OS cells using PARP1 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
PARP1 Antibody - Western blot analysis of extracts of various cell lines.
PARP1 Antibody - Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PARP1 Antibody - Western blot analysis of extracts of various cell lines, using PARP1 antibodyat 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
PARP1 Antibody - Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal PARP1 Antibody (IHC, IF, WB) LS‑C408267

Polyclonal PARP1 Antibody (IHC, IF, WB) LS‑C408267

Antibody:
Rabbit Polyclonal to Human PARP1
Application:
IHC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C408267-20
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PARP1
Application:
IHC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PARP1 antibody LS-C408267 is an unconjugated rabbit polyclonal antibody to PARP1 from human and rat. Validated for IF, IHC and WB.
Target
Human PARP1
Synonyms
PARP1 | ADPRT | ADP-ribosyltransferase NAD(+) | Adp-ribosyltransferase | ADPRT1 | ARTD1 | ADPRT 1 | Poly(ADP-ribose) synthetase | Poly(ADP-ribosyl)transferase | PARP | Poly [ADP-ribose] polymerase 1 | PADPRT-1 | PARP-1 | Poly (ADP-ribose) polymerase 1 | Poly(ADP-ribose) polymerase | Poly[ADP-ribose] synthase 1 | PPOL
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:100)
  • Immunofluorescence
  • Western blot (1:1000 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2). DQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPL
Specificity
Human PARP1
Usage
The predicted MW is 113kDa, while the observed MW by Western blot was 115kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PARP1
P09874 NM_001618 NP_001609.2

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Requested From: United States
Date Requested: 2/27/2020