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Catalog Number	Size	Price
LS-C408267-20	20 µl	$275
LS-C408267-100	100 µl	$389

PARP1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibodyat dilution of 1:100 (40x lens).

PARP1 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibodyat dilution of 1:100 (40x lens).

PARP1 Antibody - Immunohistochemistry of paraffin-embedded human mammary cancer using PARP1 antibodyat dilution of 1:100 (40x lens).

PARP1 Antibody - Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibodyat dilution of 1:100 (40x lens).

PARP1 Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer tissue.

PARP1 Antibody - Immunohistochemistry of paraffin-embedded mouse stomach tissue.

PARP1 Antibody - Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibodyat dilution of 1:100 (40x lens).

PARP1 Antibody - Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibodyat dilution of 1:100 (40x lens).

PARP1 Antibody - Immunofluorescence analysis of U2OS cells using PARP1 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.

PARP1 Antibody - Western blot analysis of extracts of various cell lines, using PARP1 antibodyat 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

PARP1 Antibody - Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

PARP1 Antibody - Western blot analysis of extracts of various cell lines.

PARP1 Antibody - Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

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Polyclonal Rabbit anti‑Human PARP1 Antibody (IHC, IF, WB) LS‑C408267

Name: Anti-PARP1 Antibody | Rabbit anti-Human Polyclonal IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C408267-20
Price: 275.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

PARP1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

$275

LS-C408267-20

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PARP1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

PARP1 antibody LS-C408267 is an unconjugated rabbit polyclonal antibody to PARP1 from human. It is reactive with human and rat. Validated for IF, IHC and WB.

Target

Human PARP1

Synonyms

See All PARP1 Antibodies

Host

Rabbit

Reactivity

Human, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2). DQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPL

Specificity

Human PARP1

Applications

IHC (1:50 - 1:100)
Immunofluorescence
Western blot (1:1000 - 1:3000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The predicted MW is 113kDa, while the observed MW by Western blot was 115kDa.

Presentation

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PARP1