Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1B11] (IgG1) to Human PARK7 / DJ-1
Western blot, ELISA
Human PARK7 / DJ-1
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1B11]
Protein G purified
Western blot (1:500 - 1:2000)
IHC - Paraffin
Specificity and Use
PARK7 / DJ-1 antibody was raised against recombinant human GST-DJ1 (1-189 aa) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
PARK7 Antibody, DJ-1 Antibody, DJ1 Antibody, Parkinson disease protein 7 Antibody, Park-7 Antibody, Oncogene DJ1 Antibody, Parkinson protein 7 Antibody, Protein DJ-1 Antibody
Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death.
Jurkat cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Park7/DJ-1 antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.