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Catalog Number Size Price
LS-B1452-50 50 µg (1 mg/ml) $485 
PARK7 / DJ-1 Antibody - Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PARK7 / DJ-1 Antibody - Anti-Human PARK7 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kD. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1300 dilution of the antibody overnight at 4C followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at RT. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
PARK7 / DJ-1 Antibody - Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PARK7 / DJ-1 Antibody - Anti-Human PARK7 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kD. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1300 dilution of the antibody overnight at 4C followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at RT. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PARK7 / DJ‑1 Antibody (C‑Terminus, IHC, WB) LS‑B1452

IHC‑plus™ Polyclonal Rabbit anti‑Human PARK7 / DJ‑1 Antibody (C‑Terminus, IHC, WB) LS‑B1452

Note: This antibody replaces LS-C18938
Antibody:
PARK7 / DJ-1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee, Zebrafish, African green monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B1452-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PARK7 / DJ-1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee, Zebrafish, African green monkey
Format:
Unconjugated, Unmodified

Specifications

Description
DJ-1 antibody LS-B1452 is an unconjugated rabbit polyclonal antibody to DJ-1 (PARK7) (C-Terminus) from human. It is reactive with human, african green monkey, chimpanzee and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PARK7 / DJ-1
Synonyms
PARK7 | DJ-1 | DJ1 | Parkinson disease protein 7 | Park-7 | Oncogene DJ1 | Parkinson protein 7 | Protein DJ-1
Host
Rabbit
Reactivity
Human, Chimpanzee, Zebrafish, African green monkey (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Zebrafish, African green monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of Human PARK7 protein.
Epitope
C-Terminus
Specificity
Cross reactivity with PARK7 from frog, mouse, rat, dog, chicken, Japanese rice fish and Atlantic salmon may also occur as the sequence varies by only one amino acid residue as indicated by BLAST analysis. Reactivity with PARK7 protein from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1452 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PARK7 / DJ-1
Q99497 NM_007262 NP_009193.2

Validation

PARK7 / DJ-1 Antibody - Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ PARK7 / DJ-1 Antibody (C-Terminus) for IHC, WB/Western, ELISA LS-B1452 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/28/2021